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Tool to cut fine samples for microscopy
A microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a cutting tool used to produce extremely thin slices of material
Microtome
The laser microtome is an instrument used for non-contact sectioning of biological tissues or materials. It was developed by the Rowiak GmbH, a spin-off
Laser_microtome
Study of the microscopic anatomy of cells and tissues of plants and animals
frozen to form hardened blocks. For light microscopy, a knife mounted in a microtome is used to cut tissue sections (typically between 5-15 micrometers thick)
Histology
Cooling device
called a microtome, placed in a freezer. The cryostat is usually a stationary upright freezer, with an external wheel for rotating the microtome. The temperature
Cryostat
Marrow puncture Bone marrow biopsy needle Rotary microtome Electrical microtome Base sledge microtome Hypodermic needle Intravenous cannula Peak flow meter
Instruments_used_in_pathology
Swiss anatomist (1831–1904)
invented the microtome. By treating animal tissue with acids and salts to harden it and then slicing it very thinly with the microtome, scientists were
Wilhelm_His_Sr.
Genus of moths
Microtome is a genus of moths in the family Geometridae. Pitkin, Brian & Jenkins, Paul (2023). "Search results Family: Geometridae". Butterflies and Moths
Microtome_(moth)
Process in medicine
compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections)
Optimal cutting temperature compound
Optimal_cutting_temperature_compound
Scottish watchmaker and inventor (1733–1814)
The Climate of London. In 1770 he is credited with the invention of the microtome, a machine for making extremely thin slices as used in slide-preparation
Alexander_Cumming
Partial or complete separation of a body or system into two or more parts
cutting are the knife and saw, or in medicine and science the scalpel and microtome. However, any sufficiently sharp object is capable of cutting if it has
Cutting
Rapid histological sectioning procedure
oncological surgery. The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome
Frozen_section_procedure
Equation relating the concentration of a component and surface tension
surface tension concentration data and the Gibbs adsorption equation. The microtome blade method is used to determine the weight and molal concentration of
Gibbs_isotherm
Academic journal
editions of the journal were published by MIT Press until 2004 and by Microtome Publishing thereafter. From its inception, the journal received no revenue
Journal of Machine Learning Research
Journal_of_Machine_Learning_Research
Method of 3D bioimaging
is that the thickness of the slice which can be removed with the ultra-microtome is limited (~25 nm), thus the resolution in the depth direction is limited
Serial block-face scanning electron microscopy
Serial_block-face_scanning_electron_microscopy
German scientific instruments manufacturer
1872, as Precision Engineering Headquarters Nussloch, Germany Products Microtome; Cryostat; Vibratome, Digital Pathology Scanner Parent Danaher Corporation
Leica_Biosystems
Microscopic examination of tissue in order to study and diagnose disease
provide a properly oriented sample sturdy enough for obtaining a thin microtome section(s) for the slide. Once the wax embedded block is finished, sections
Histopathology
Study of the structure of organisms
study of small structures involved passing light through them, and the microtome was invented to provide sufficiently thin slices of tissue to examine
Anatomy
under electron microscopy, sections should be from 20 to 30 nanometers. Microtome can be used in sectioning of sufficiently thin slices. If the objects
Microtechnique
Histological staining method
embedded in melted paraffin wax, the resulting block is mounted on a microtome and cut into thin slices. The slices are affixed to microscope slides
H&E_stain
Soft colorless solid derived from petroleum, coal or shale oil
set in a mold with wax to cool and solidify. Sections are then cut on a microtome. Anti-caking agent, moisture repellent, and dustbinding coatings for fertilizers
Paraffin_wax
small slivers of wood for examination under a microscope, often using a microtome. It is useful for providing forensic evidence in some criminal cases where
Xylotomy
Refractive eye surgery procedure
regenerative capacity. The deeper layers, if reshaped by a laser or cut by a microtome, will remain that way permanently with only limited healing or remodelling
Photorefractive_keratectomy
Microscopic study of the anatomy of human nervous tissue
the ways to parse the brain, by obtaining sections of the brain using a microtome and staining them with chemical agents which reveal where different neurons
Cytoarchitecture
Canadian-American cell biologist (1912–1997)
fibrin, collagen, T-tubules and sarcoplasmic reticulum. He also introduced microtome cutting. Keith Porter was born in Yarmouth, Nova Scotia, on June 11, 1912
Keith_R._Porter
List of academic journals in artificial intelligence
Learning (journal) – Springer Journal of Machine Learning Research – Microtome Pattern Recognition (journal) – Elsevier Neural Networks (journal) – Elsevier
List of artificial intelligence journals
List_of_artificial_intelligence_journals
Culinary utensil used for slicing food
deli products. Microplane – used for the grating of various food items. Microtome – the laboratory-grade equivalent, for much finer slicing thicknesses
Mandoline
Science and craft of managing woodlands
Hemispherical photography Inclinometer Increment borer Rangefinder laser Microtome Relascope Tree caliper Wedge prism Fire suppression Aerial firefighting
Forestry
Programming language that uses first order logic
Pereira; Stuart M. Shieber (2005). Prolog and Natural Language Analysis. Microtome. Colmerauer, A. and Roussel, P., 1996. The birth of Prolog. In History
Prolog
Type of material
microscope samples in plastic so they may be sectioned (sliced thin) with a microtome and then imaged. Epoxy resin, mixed with pigment, may be used as a painting
Epoxy
Chemical compound
used as a decalcifying agent making it possible to cut sections using a microtome once the tissue sample is demineralised. EDTA is also known to inhibit
Ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic_acid
Type of electron microscope
before examination in the microscope. Samples may be sectioned (with a microtome) if information about the organism's internal ultrastructure is to be
Scanning_electron_microscope
Coding of traded products by the World Customs Organization
checking quantities of heat, sound or light (including exposure meters); microtomes). If the CO detector does not capture and display gas measurements, then
Harmonized_System
Canadian eye health company
company also produced photographic lenses (1883), spectacle lenses (1889), microtomes (1890), binoculars and telescopes (1893). From 1892 in cooperation with
Bausch_&_Lomb
Czech biologist and physiologist (1787–1869)
protoplasm (the substance found inside cells.) Purkyně was the first to use a microtome to make thin slices of tissue for microscopic examination and was among
Jan_Evangelista_Purkyně
Microscopic feature of cardiac muscle
intercalated discs, when seen on histopathology, have two main causes: Microtome sectioning, thereby being a visual artifact. Forceful myocardial contraction
Intercalated_disc
Silver staining technique for visualizing nervous tissue under light microscopy
particles of gold. Golgi staining has traditionally been performed on microtome-prepared sections, while its use in cryosectioning has been limited. Modified
Golgi's_method
Aerosol spray for rapid cooling
section biopsies to rapidly cool or freeze small tissue samples at the microtome or cryostat. Freeze spray has been shown useful for the field marking
Freeze_spray
Imaging and diffraction using electrons that pass through samples
(1961). "Diamond knife ultra microtomy of metals and the structure of microtomed sections". British Journal of Applied Physics. 12 (10): 554. Bibcode:1961BJAP
Transmission electron microscopy
Transmission_electron_microscopy
Knife in which the edge is made from diamond
astigmatism and cure the first and second stages of keratoconus. Metal microtome knives or razor blades are too soft and dull to cut ultrathin sections
Diamond_knife
paraffin or frozen medium. The cut edge is then thinly sliced with a microtome or a cryostat. The thin slices are then mounted on a glass slide, stained
Bread_loafing
Medical nonprofit in Utica, United States
fresh frozen and fixed frozen tissue sectioning Leica RM2125 RTS manual microtome – for paraffin embedded tissue sectioning Keyence BZ-X800 – an “all-in-one”
Masonic Medical Research Institute
Masonic_Medical_Research_Institute
Anatomist and histologist from the Russian Empire (1834–1894)
fixation and staining (particularly with carmine). He invented an original microtome (a "histological guillotine") that allowed him to cut remarkably large
Vladimir_Betz
(2011). "Waffles: A Machine Learning Toolkit" (PDF). Journal of Machine Learning Research. 12 (1532–4435). JMLR.org and Microtome Publishing: 2383–2387.
Waffles_(machine_learning)
Thin, flat piece of glass onto which a sample is placed to be examined under a microscope
replacing the water with paraffin, cutting it into very thin sections using a microtome, placing the sections on a microscope slide, staining the tissue using
Microscope_slide
Laboratory method involving brain slices
limitation and difficult to observe progress. Modern microtome devices such as Compresstome microtomes are used to prepare slices as these devices have less
Slice_preparation
Skin surgery
removed circumferentially around the tumour and sectioned with a cryostat microtome. This is followed by tissue processing and viewing under a microscope
Dermatologic surgical procedure
Dermatologic_surgical_procedure
Darwin Barlow was later chairman. Designed between 1883/84, the rocking microtome, otherwise known as The "Darwin Rocker", was one of Darwin's most successful
Cambridge Scientific Instrument Company
Cambridge_Scientific_Instrument_Company
Imaging of focal planes within a thick sample
to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several
Optical_sectioning
Junior college in West Central, Tainan, Taiwan
Research facilities and equipment such as human tissue slides, modules, microtome, flow cytometry, and protein 2D-system. The division also hosts and annual
National Tainan Junior College of Nursing
National_Tainan_Junior_College_of_Nursing
cannot be seen by the naked eye. Microtitre plates mostly used for ELISA Microtome cuts prepared specimens for analysis under microscope Nichrome wire loop
Instruments used in microbiology
Instruments_used_in_microbiology
Electrical properties of biological cells
delicate slicing devices like the compresstome vibratome, leica vibratome, microtome are often employed. These instruments aid in obtaining precise, thin brain
Electrophysiology
Natural stain derived from hearthwood and used in histology
Bracegirdle, Brian (1986). A history of microtechnique : the evolution of the microtome and the development of tissue preparation (2nd ed.). Lincolnwood, IL:
Haematoxylin
Humberto Fernández-Morán (1924–1999), Venezuela – Diamond scalpel, Ultra microtome Michele Ferrero (1925–2015), Italy – Kinder Surprise = Kinder Eggs, Nutella
List_of_inventors
Decalcification is necessary to obtain soft sections of the bone using a microtome. Every thin section of the bone that is cut can be processed (see tissue
Bone_decalcification
German laboratory equipment company
(Göttingen) and Ludwig Tesdorpf (Stuttgart). These companies manufactured microtomes, as well as astronomical, geodetic, and physical instruments. In 1969
Sartorius_AG
Laboratory technique in electrophysiology
membrane voltage. Accurate tissue sectioning with compresstome vibratome or microtomes is essential, in addition to patch clamp methods. By supplying thin, uniform
Patch_clamp
American engineer
Bell company for many years. Blake also improved the construction of the microtome, and photographic shutter. Blake worked on the United States Coast Survey
Francis_Blake_(inventor)
microscope samples in plastic so they may be sectioned (sliced thin) with a microtome and then imaged. Durcupan is notable for refractive index nD20 of 1.654
Durcupan
Family of related stains for examination of blood
Bracegirdle, Brian (1986). A history of microtechnique : the evolution of the microtome and the development of tissue preparation (2nd ed.). Lincolnwood, IL:
Romanowsky_stain
Indian botanist and writer
embryology of orchids at home after obtaining a second-hand microscope, a microtome and some basic laboratory tools. He received a PhD from the University
B._G._L._Swamy
Common application of immunostaining
formalin can be used. Sectioning of the tissue sample is done using a microtome. For paraffin embedded tissue 4 μm is normal thickness, and for frozen
Immunohistochemistry
Technique used to enhance visual contrast of specimens observed under a microscope
For larger pieces of tissue, thin sections (slices) are made using a microtome; these slices can then be mounted and inspected. Most of the dyes commonly
Staining
British economic botanist (1878–1977)
agricultural college but this was a failure. He helped develop a rocking microtome and a seed-drill which he patented. Martin-Leake left India in 1923, and
Hugh_Martin-Leake
Species of virus
diagnostic procedure. Sections of fetal tissues are prepared with a cryostat microtome and are then reacted with standardized reagents. The test can be completed
Porcine_parvovirus
German physician and scientist (1854–1915)
schoolboy (inspired by his cousin Karl Weigert who owned one of the first microtomes), he became fascinated by the process of staining microscopic tissue substances
Paul_Ehrlich
Species of tree native to South Florida and islands in the Caribbean
and cases for delicate apparatuses such as scales, microscopes, and microtomes. Swietenia has a long history of being used to make boxes and cases for
Swietenia_mahagoni
American microbiologist
Nocardia. After retiring from teaching, she focused on using a precision microtome to slice through bacterial cells and investigated the internal structure
Margaret_Hotchkiss
American optician and businessman
company also produced photographic lenses (1883), spectacle lenses (1889), microtomes (1890), binoculars and telescopes (1893). The firm was incorporated as
John_Jacob_Bausch
Swiss myrmecologist, neuroanatomist and psychiatrist (1848–1931)
various techniques in neuro-anatomy including modifications to Gudden's microtome design. Forel had a diverse and mixed career as a thinker on many subjects
Auguste_Forel
Venezuelan scientist (1924–1999)
Square Luxburg-Carolath in his hometown, Maracaibo. Diamond knife Ultra microtome 1967, the John Scott Award, for his invention of the diamond scalpel.
Humberto_Fernández-Morán
Scottish surgeon and rugby union player
wartime service. Cathcart had a flair for mechanical design. He designed a microtome for frozen section, a technique which had only recently been introduced
Charles_Walker_Cathcart
Hemispherical photography Inclinometer Increment borer Rangefinder laser Microtome Relascope Tree caliper Wedge prism Fire suppression Aerial firefighting
Wedge_prism
observed in cells which have been homogenized. microspike See filopodium. microtome An instrument used to cut extremely thin slices of material, known as
Glossary of cellular and molecular biology (M–Z)
Glossary_of_cellular_and_molecular_biology_(M–Z)
Science Museum in Leiden, Netherlands
22 Medicine: Man as a machine Pieces at display include: a gigantic microtome, the first heart-lung machine (prototype) by inventor Jacob Jongbloed
Museum_Boerhaave
Saw mounted on a long pole, usually for pruning trees
Hemispherical photography Inclinometer Increment borer Rangefinder laser Microtome Relascope Tree caliper Wedge prism Fire suppression Aerial firefighting
Polesaw
Large band saw
Hemispherical photography Inclinometer Increment borer Rangefinder laser Microtome Relascope Tree caliper Wedge prism Fire suppression Aerial firefighting
Resaw
British pathologist (1798–1866)
Bracegirdle, Brian (1986). A history of microtechnique: the evolution of the microtome and the development of tissue preparation. Science Heritage Ltd. pp. 27–
Thomas_Hodgkin
Preparation and study of ceramics with optical instruments
~1 mm thick, glued to a microscope slide, and ground or sawed (e.g., by microtome) to a thickness (x) approaching 30 μm. A cover slip is glued onto the
Ceramography
German neuroanatomist and psychiatrist
Gudden in his honor, and he is credited for developing a specialized microtome for sectioning the brain for pathological study. Among his well-known
Bernhard_von_Gudden
Tissue arrangement technique
precisely spaced, array pattern. Sections from this block are cut using a microtome, mounted on a microscope slide and then analyzed by any method of standard
Tissue_microarray
Public university in Pavia, Italy
of his scientific publications and instruments used such as syringes, microtomes, microscopes, original photographic plates of histological preparations
University_of_Pavia
Three-dimensional imaging technique
blocks of cells or tissue are sectioned into thin samples with a cryo-microtome. In FIB-milling, plunge-frozen samples are exposed to a focused beam of
Cryogenic_electron_tomography
Israeli physician
necessary to cut thinner slices of tissue. Thus, David Danon developed the Microtome (a device for cutting extremely thin slices of tissue) for use on the
David_Danon
Thin sections of lung tissue used in respiratory research
advancement occurred in the 1940s when Stadie and Riggs introduced a microtome equipped with a thin razor blade, reducing thickness variability to about
Precision_cut_lung_slices
Staining technique used in electron microscopy
technique. First, a thin section of the sample is cut, often using a microtome. Various other stages of sample preparation may then take place. The prepared
Immunogold_labelling
labelling. For EM imaging, the sample may be cut into thin slices with a microtome, then imaged using transmission electron microscopy. This method is commonly
Neural_circuit_reconstruction
Machine for cutting food or materials
paper or silver. In science, types of cutting machines used are called microtomes. Meat slicer Industrial bread cutting machine Mechanic bread cutting machine
Cutting_machine
Effects from freeze and thaw cycles
he used a microtome in a vacuum chamber to fracture the specimens. Much cheaper, non-commercial alternatives which did not rely on a microtome or etching
Freeze-fracture
British surgeon
particularly cancer of the breast. Cheatle designed an exceptionally large microtome that could cut 10 inches (250 mm) square sections, with which his technician
George_Lenthal_Cheatle
involved in the control of internal organs. Wilhelm His, Sr., inventor of microtome Albert Hofmann, inventor of LSD Paul Karrer, (nobel prize), research on
List of Swiss inventors and discoverers
List_of_Swiss_inventors_and_discoverers
paraffin and sliced into 7,404 20 μm thick sections using a large-scale microtome. After each section was removed, the uncut face was photographed in order
BigBrain
Spanish civil engineer (1852–1936)
acquired a stalagmometer; and Santiago Ramón y Cajal commissioned both a microtome and a panmicrotome, alongside a film projector. The development of the
Leonardo_Torres_Quevedo
Human-based computation game
bring out the contrast of the plasma membranes, sliced into layers by a microtome, and imaged using an electron microscope. A number of in-progress neurons
Eyewire
Microlyces Microlygris Micromia Micronidia Micronissa Microplutodes Microsema Microtome Microxena Microxydia Micrulia Mictodoca Mictoschema Middletonia Milionia
List_of_geometrid_genera:_M
American physicist
History. The Optical Society. Retrieved 25 October 2013. Microscopes, Microtomes, Colorimeters, Optical Measuring Instruments and Accessories. Bausch &
Charles_S._Hastings
of the second world war, MSE was retained by them to develop hospital Microtomes, which until then had not been manufactured in the UK. In the early 1950s
MSE_(centrifuges)
Formal means of expressing grammar
F. C. N.; S. M. Shieber (2002). Prolog and natural-language analysis. Microtome Publishing. ISBN 9780971977709. Fleck, Arthur. "Definite Clause Grammar
Definite_clause_grammar
Type of sawmill incorporating a chainsaw
Hemispherical photography Inclinometer Increment borer Rangefinder laser Microtome Relascope Tree caliper Wedge prism Fire suppression Aerial firefighting
Chainsaw_mill
English biologist (1924–2013)
innovative contribution was making a modified version of thin-sectioning microtome, by which he could make histological sections of only 100–150 Å in thickness
Hugh_Huxley
British surgeon (1863–1946)
the operating theatre by an expert pathologist armed with a freezing microtome." Stiles was the first surgeon to transplant the ureter into the sigmoid
Harold_Stiles
MICROTOME
MICROTOME
MICROTOME
MICROTOME
Girl/Female
Christian & English(British/American/Australian)
The Holly Bush
Boy/Male
Hindu, Indian
Graceful
Girl/Female
American, Australian, Christian
Combination of Rose and Anne
Boy/Male
Shakespearean
King Henry IV, Part 1 and 2' Edward Poins, an irregular humorist.
Boy/Male
Hindu
Lord venkateswara
Female
Yiddish
Variant spelling of Yiddish Sheina, SHAYNA means "beautiful."
Girl/Female
Muslim
Castle
Girl/Female
Muslim/Islamic
Acquirer obtainer, winner
Surname or Lastname
English (southern)
English (southern) : from Old French poulain ‘colt’; a metonymic occupational name for a horse-breeder or nickname for a frisky person.
Boy/Male
Australian, Celtic, Irish
Strong in Battle; Battle Strong
MICROTOME
MICROTOME
MICROTOME
MICROTOME
MICROTOME
n.
An instrument for making very thin sections for microscopical examination.
n.
The art of using the microtome; investigation carried on with the microtome.