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Cross-linked dextran gel used for gel filtration
Sephadex is a cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin
Sephadex
Chromatography process
the negative charge of the substance. A slurry of resin, such as DEAE-Sephadex is poured into the column. The matrix that is used is insoluble with charged
Anion-exchange_chromatography
Chemical compound
Pregnenolone product can be separated from cholesterol substrate using Sephadex LH-20 minicolumns. Pregnenolone is lipophilic and readily crosses the blood–brain
Pregnenolone
Chemical-analysis technique
(kDa) 1 Sephadex G-10 0–0.7 2 Sephadex G-25 1–5 3 Sephadex G-50 1.5–30 4 Sephadex G-75 3–70 5 Sephadex G-100 4–150 6 Sephadex G-150 5–300 7 Sephadex G-200
Size-exclusion_chromatography
Laboratory technique to separate ions and polar molecules
from a positive side group of diethylaminoethyl bound to cellulose or Sephadex. Agarose gel based medium contain large pores as well but their substitution
Ion_chromatography
Surface on which a plant or animal lives
Monolayer of cell must be grown. Moreover, plastic beads of polystyrene, sephadex and polyacrylamide are also available for cell growth in suspension culture
Substrate_(biology)
German-American molecular biologist (1936–2026)
of California, Berkeley Thesis Fraktionierungen von Proteinen – besonders Enzymen – mit Ionenaustauschern und Molekularsieben <vom Typ Sephadex> (1963)
Peter_Duesberg
Sterol biosynthesized by all animal cells
purify cholesterol prior to use. Cholesterol can be purified using small Sephadex LH-20 columns. Cholesterol is oxidized by the liver into a variety of bile
Cholesterol
Chemical compound
epichlorohydrin is its use as crosslinking agent for the production of Sephadex size-exclusion chromatographic resins from dextrans. Epichlorohydrin is
Epichlorohydrin
Chemical compound
also used in some size-exclusion chromatography matrices; an example is Sephadex. Dextran has also been used in bead form to aid in bioreactor applications
Dextran
Type of size-exclusion chromatography
Styragel (cross-linked polystyrene-divinylbenzene), LH-20 (hydroxypropylated Sephadex), Bio-Gel (cross-linked polyacrylamide), HW-20 & HW-40 (hydroxylated methacrylic
Gel_permeation_chromatography
resin through lysine side chains. Gel permeation chromatography Superose Sephadex Ahmed, Hafiz (2004). Principles and Reactions of Protein Extraction, Purification
Sepharose
Method to detect an antigen using an antibody and enzyme
Leif; Porath, Jerker (1966). "Radioimmunoassay of proteins with the use of Sephadex-coupled antibodies". Biochimica et Biophysica Acta (BBA) - General Subjects
ELISA
Pharmaceutical and biotechnological company
head of research. In 1959, Pharmacia pioneered gel filtration with its Sephadex products. These were also based on dextran and discoveries in Tiselius'
Pharmacia
Species of centipede
identified from the venom of S. mutilans. These peptides were identified by Sephadex gel filtration and RP-high-performance liquid chromatography. Both scolopin
Chinese_red-headed_centipede
Swedish biochemist (1921–2016)
on 13 June 1959. A short time thereafter, Pharmacia offered the product Sephadex (Separation Pharmacia Dextran) on the market. Porath also worked on affinity
Jerker_Porath
Medical products company
Porath, then presented their ideas to use Pharmacia's separation medium Sephadex as the solid phase in immunodiagnostics tests and proposed a collaboration
Phadia
Proteolytic enzyme that digests keratin
disulphide cross-linked thiolated macromolecules within cross-linked dextran ("Sephadex") gels". Journal of Pharmacy and Pharmacology. 33 (1): 410–411. doi:10
Keratinase
of the apamin-competing fraction of the venom from the scorpion via a Sephadex G-50 size exclusion chromatography, followed by high performance liquid
Tamapin
Protein complex for mRNA transcription
was obtained by the use of ion-exchange chromatography via DEAE coated Sephadex beads. The technique separated the enzymes by the order of the corresponding
RNA_polymerase_II
Resin used in ion-exchange chromatography
for various molecular biology studies. DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. DEAE-C is a weak anion exchanger.
Diethylaminoethyl_cellulose
Chemical compound
potassium hydroxide (KOH) and purified by size-exclusion chromatography on Sephadex LH using DMSO–0.1%TFA. The fine-structure of the ultraviolet-visible absorption
Granadaene
Cyclic peptide part of a group of toxins present in Amanita mushrooms
toxins were then desalted and taken through four different methods of sephadex and acidic protein purification. The structure of β-Amanitin was determined
Β-Amanitin
Enzyme
"Purification of rabbit intestinal glucoamylase by affinity chromatography on Sephadex G-200". Indian J. Biochem. Biophys. 10 (4): 283–284. PMID 4792946. Sørensen
Α-Glucosidase
Family of enzymes
separation in the Y fraction (as opposed to the "X and Z" fractions) using Sephadex G75 chromatography. As GST sub-units were identified they were referred
Glutathione_S-transferase
the growth of anchorage-dependent cells, and he used diethylaminoethyl–Sephadex microcarriers. However, synthetic polymers prevent sufficient cell interactions
Microcarrier
American chemist (born 1934)
19640680818. Fasella, Paolo; Hammes, Gordon G.; Schimmel, Paul R. (1965). "A Sephadex dialysis method of determining small molecule-macromolecule binding constants"
Gordon_Hammes
Chemical separation technique
commonly used supporting matrix would be cross-linked agarose (sepharose), sephadex, polyacrylamide, and silica. An example for triazine linkage immobilization
Dye-ligand affinity chromatography
Dye-ligand_affinity_chromatography
Chemical compound
venom is dissolved in ammonium acetate buffer and then loaded on the CM-Sephadex column. The elution of the compound is done in two different steps by using
Α-Bungarotoxin
loop allows the injection of the desired sample. Size exclusion Sepharose Sephadex Andersson, T et al. Agarose-based media for high-resolution gel filtration
Superose
Fricke dosimeter prepared using a gelling agent
Fricke solutions with various gelling agents such as gelatine, agarose, sephadex and polyvinyl alcohol (PVA) were investigated along with chelating agents
Gel_dosimetry
Snake toxin
alpha-neurotoxin-like peptide can be separated from the protease inhibitor, Sephadex G-50 gel filtration chromatography can be used, in the presence of high
Taicatoxin
Chemical compound
crystallization. Initial purification uses size-exclusion chromatography with Sephadex LH20 (a size-exclusion resin) in a dichloromethane-acetone system to separate
Protolichesterinic_acid
Chemical compound
solid media, AD2. The methyl ethyl ketone extract of the fungal growth on Sephadex LH 20 (a liquid chromatography medium designed for the separation of small
Integrasone
Blood glucose measurement device
(fluorescent). To improve the stability they attached the protein to a sephadex, a macroporous hydrogel. Despite the change in Fret of only 35% across
Fluorescent_glucose_biosensor
Type of glycoprotein
and the supernatant was freeze-dried. The extract was fractioned using a Sephadex column. The different fractions were assayed for toxicity to mice, to which
Modeccin
Column used in high-performance liquid chromatography
The first gels for use in LC were created using cross-linked dextrans (Sephadex) in an attempt to realize Synge's prediction that a unique single-piece
Monolithic_HPLC_column
SEPHADEX
SEPHADEX
SEPHADEX
SEPHADEX
Boy/Male
Hindu
Famous, Renowned
Girl/Female
Arabic, Gujarati, Hindu, Indian, Kannada, Marathi, Muslim
Faithful
Surname or Lastname
English
English : occupational name for a layer of paving, from Middle English, Old French pavier ‘paver’, an agent derivative of Old French paver ‘to pave’ (though the Old French verb may be a back-formation from pavement ‘laid floor’).
Boy/Male
American, British, English, Gaelic, Irish
Great
Boy/Male
Greek
Gentle. To tame. A. In Greek legend Damon was a loyal friend of Pythias. Famous bearer in modern...
Girl/Female
American, Assamese, Christian, Danish, French, German, Hindu, Indian, Italian, Japanese, Kannada, Latin, Malayalam, Marathi, Punjabi, Sikh, Sindhi, Spanish, Telugu
Joy; Quiet; Calm; Peaceful; Sunlight
Girl/Female
Indian, Telugu
Like History
Boy/Male
Australian, Vietnamese
Prosperous; Developed
Girl/Female
Arabic, Muslim
Brilliance
Girl/Female
Indian, Tamil
Soft
SEPHADEX
SEPHADEX
SEPHADEX
SEPHADEX
SEPHADEX