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Chemical test for detecting peptide bonds
In chemistry, the biuret test (IPA: /ˌbaɪjəˈrɛt/, /ˈbaɪjəˌrɛt/), also known as Piotrowski's test, is a chemical test used for detecting the presence of
Biuret_test
Chemical compound
Biuret (/ˈbjurɛt/ BYUR-ret) is a chemical compound with the chemical formula HN(CONH2)2. It is a white solid that is soluble in hot water. A variety of
Biuret
Stomach acid suppressing medication
Lansoprazole, sold under the brand name Prevacid among others, is a medication which reduces stomach acid. It is a proton pump inhibitor (PPI), used to
Lansoprazole
Organic compound
CO(NH2)2 → [NH4]+[OCN]− → NH3 + HNCO Heating above 160 °C (320 °F) yields biuret NH2CONHCONH2 and triuret NH2CONHCONHCONH2 via reaction with isocyanic acid:
Urea
Method to determine protein concentration
to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change
Bicinchoninic_acid_assay
Class of enzymes
Biuret amidohydrolase (EC 3.5.1.84) is an enzyme characterised from bacteria that catalyzes the hydrolysis of biuret to allophanic acid and ammonia: biuret
Biuret_amidohydrolase
Chemical compound
precipitant in protein crystallography and is also an ingredient in the Biuret reagent which is used to measure protein concentration. This ingredient
Potassium_sodium_tartrate
Chemical compound belonging to the class of triazine
streams. Intermediates in the dehydration include both isocyanic acid, biuret, and triuret: H2N-CO-NH2 → HNCO + NH3 H2N-CO-NH2 + HNCO → H2N-CO-NH-CO-NH2
Cyanuric_acid
Reagent used to detect water soluble proteins
other compounds containing the phenol functional group. Therefore, the biuret test or the ninhydrin reaction are used along with it to confirm the presence
Millon's_reagent
Chemical compound
H2NC(O)NHCO2H. It is a carbamic acid, the carboxylated derivative of urea. Biuret can be viewed as the amide of allophanic acid. Treating urea with sodium
Allophanic_acid
Parameter representing the concentration of protein
protein type. The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as dye-binding and refractometry
Serum_total_protein
Industrial urea manufacturing process
stripper, so residence times in these stages are designed to be short. Biuret is formed when two molecules of urea combine with the loss of a molecule
Bosch–Meiser_process
Chemist and entrepreneur
for ruminant animals made from newsprint, and a method for synthesizing biuret for use in animal feed, among hundreds of other patents. His wife, Edna
Jonas_Kamlet
Chemical compound
detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single
Bicinchoninic_acid
Biochemical colorimetric method
reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method
Lowry_protein_assay
Chemical compound
acid beta-Ureidoisobutyric acid N-Acetylaspartic acid Related compounds Biuret Bromisoval Carbromal Except where otherwise noted, data are given for materials
Carbamoyl_aspartic_acid
German-Italian chemist (1834–1915)
of the Schiff test. He also worked in the field of amino acids and the Biuret reagent. Born in Frankfurt am Main, Schiff was a student of Friedrich Wöhler
Hugo_Schiff
Class of enzymes
cyanuric acid, which proceeds via biuret and this compound is also hydrolysed, although more slowly than allophanic acid. Biuret is a minor component in commercial
Allophanate_hydrolase
Chemical compound
3-Ureidopropionic acid Carbamoyl aspartic acid N-Acetylaspartic acid Related compounds Biuret Bromisoval Carbromal Except where otherwise noted, data are given for materials
Β-Ureidoisobutyric_acid
Chemical compound
(NH4)2CO3 The reaction produces a liquid. Intermediates and impurities include biuret, cyanuric acid, and potassium allophanate (KO2CNHC(O)NH2), as well as unreacted
Potassium_cyanate
Chemical compound
to insoluble red copper(I) oxide. Copper(II) sulfate is also used in the Biuret reagent to test for proteins. Copper sulfate is used to test blood for anemia
Copper(II)_sulfate
Branch of biology that studies biological systems at the molecular level
quantitation compared to previous methods: the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not susceptible
Molecular_biology
South Korean musical duo
— "Everyone's Breath" (숨소리; Sumsori) (with Bye Bye Sea, Romantic Punch, Biuret, Super Kidd, April 2, Cold Cherry, Hash, Haze Moon Band, and T.A-Copy) 2017
Seenroot
German chemist (1852–1919)
responses to tests for proteins used by physiological chemists - a positive Biuret test, precipitation by inorganic salts and cleavage by proteolytic enzymes
Emil_Fischer
Method of determining chemical concentration
a chemical reaction to determine protein concentration. The Biuret assay utilizes a biuret reagent which turns purple in the presence of proteins due to
Colorimetric_analysis
Chemical group (–N=C=O)
+ R'NCO → R2NC(O)N(H)R' The addition of an isocyanate to a urea gives a biuret: R2NC(O)N(H)R' + R''NCO → R2NC(O)NR'C(O)NHR'' Reaction between a di-isocyanate
Isocyanate
Linear sequence of amino acids in a peptide or protein
Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was followed a few hours later by Emil
Protein_primary_structure
Chemical compound
the condensation reaction among 2 moles of dicyandiamide and 1 mole of biuret. 2 C2H4N4 + C2H5N3O2 → 2C3H5N5O + NH3 Ammeline is weakly acidic with pKa
Ammeline
Rock music from South Korea
Line Butterfly 9 and the Numbers Achime Add 4 Apollo 18 Autumn Vacation Biuret Bluedawn Bongjeingan Boohwal Broccoli, You Too? Bulldog Mansion Bulssazo
Korean_rock
Chemical compound
beta-Ureidoisobutyric acid Carbamoyl aspartic acid N-Acetylaspartic acid Related compounds Biuret Bromisoval Carbromal Except where otherwise noted, data are given for materials
3-Ureidopropionic_acid
Chemical compound
to aircraft and vessels. HDI is also sold oligomerized as the trimer or biuret which are used in automotive refinish coatings. Although more viscous in
Hexamethylene_diisocyanate
South Korean annual music festival held in Busan
8 August Unchained GumX Biuret Trans Fixion Neil Zaza Crash Soilwork Arch Enemy 9 August Discotruck Sugar Donut Lazybone Down In A Hole Wiretrap In My
Busan_Rock_Festival
Chemical compound
complicating by-product in the industrial synthesis of melamine from urea. Urea Biuret Cyanuric acid International Union of Pure and Applied Chemistry (2014).
Triuret
Method to determine protein concentration
Alternative protein assays include: Ultraviolet–visible spectroscopy RGBradford Biuret protein assay Lowry protein assay BCA protein assay Amido black protein
Bradford_protein_assay
Chemical compound
Dithiobiuret is extremely toxic; exposure can result in respiratory failure. Biuret Spofford, W. A.; Amma, E. L. (1972). "Crystal and molecular structure of
Dithiobiuret
planar with a bent M-O-C angle. Biuret (H2NC(O)N(H)C(O)NH2) is a derivative of urea but with two amido groups. Biuret forms a variety of metal complexes
Transition metal carboxamide complex
Transition_metal_carboxamide_complex
South Korean annual music festival held in Incheon
Galaxy Express Lun Hui Hoobastank LCD Soundsystem YB Kishidan Guckkasten Biuret Ian Brown Dir En Grey Kim Chang-wan Ego-Wrappin' Lee Han-Chul Huckleberry
Pentaport_Rock_Festival
without replacement] ISO 2754:1973 Urea for industrial use — Determination of biuret content — Photometric method [Withdrawn without replacement] ISO 2755:1973
List of ISO standards 2000–2999
List_of_ISO_standards_2000–2999
Analytical chemistry technique
accurate spectrophotometric procedures for protein quantification include the Biuret, Lowry, BCA, and Bradford methods. An alternative method for label free
Quantitative_proteomics
Overview of and topical guide to organic chemistry
annulation Robinson annulation Barfoed's test Bayer test Benedict's test Biuret test Fehling's test Molisch's test Ninhydrin test Schiff test Seliwanoff's
Outline_of_organic_chemistry
German chemist (1850–1922)
for peptide bonds based on the biuret reaction observed with all proteins but never with free amino acids. Since biuret has the formula NH2-CO-NH-CO-NH2
Franz_Hofmeister
Class of enzymes
1-carboxybiuret, is unstable and can spontaneously decarboxylate to give biuret. This enzyme is a hydrolase, one acting on carbon-nitrogen bonds other than
Cyanuric_acid_amidohydrolase
used in animal nutrition to refer collectively to components such as urea, biuret, and ammonia, which are not proteins but can be converted into microbial
Nitrogen and Non-Protein Nitrogen's effects on Agriculture
Nitrogen_and_Non-Protein_Nitrogen's_effects_on_Agriculture
reduction Bischler–Möhlau indole synthesis Bischler–Napieralski reaction Biuret test Blaise ketone synthesis Blaise reaction Blanc reaction Blanc chloromethylation
List_of_organic_reactions
Index of chemical compounds with the same molecular formula
C2H5N3O2 (molar mass: 103.08 g/mol, exact mass: 103.0382 u) may refer to: Biuret N-Nitroso-N-methylurea (NMU) This set index page lists chemical structure
C2H5N3O2
Arab Muslim ophthalmologist during the period of medieval Islam
معجم الأدباء 1-7 من العصر الجاهلي حتى سنة 2002م (in Arabic). Vol. fifth. Biuret, Lebanon: دار الكتب العلمية. p. 310. Archived from the original on 24 September
Aayon_Ibn_Aayon
Procedure for identifying or quantifying a chemical compound or group
glucose and fructose The bicinchoninic acid assay tests for proteins The Biuret test tests for proteins and polypeptides Bradford protein assay measures
Chemical_test
Class of organic compound
controlled as the isocyanate intermediate can react with the urea to form a biuret: R2NC(O)N(H)R' + R"NCO → R2NC(O)NR'C(O)NHR" Unsymmetrical ureas are generated
Ureas
Food contamination events in China
given to ruminants in the form of pelleted urea, ammonium phosphate and/or biuret. Sometimes slightly polymerized special urea-formaldehyde resin or a mixture
Protein_adulteration_in_China
number unknown] ISO 4274:1977 Urea for industrial use — Determination of biuret content — Flame atomic absorption and photometric absorption methods [Withdrawn
List of ISO standards 3000–4999
List_of_ISO_standards_3000–4999
Musical artist
winning the K-Pop India Contest, Techi toured with the Korean pop-rock band BIURET on their maiden visit to India. While on Tour, her appearances at Music
Boby_Techi
2026 spelling competition
mentis catometope Faesulae Bhubaneswar vaesite cour d'honneur euphausid biuret oconee bells Ceutorhynchus potto kurgan sambaqui cere raguly Pluchea cydnid
98th Scripps National Spelling Bee
98th_Scripps_National_Spelling_Bee
Waldorf Thomas Thomas, A. W. (1913). A further effort to prepare a colorless biuret reagent. Biochemical Bulletin (New York), 2, 556–8. Thomas, A. W. (1914)
Arthur_W._Thomas
N-acyl-D-glutamate deacylase EC 3.5.1.83: N-acyl-D-aspartate deacylase EC 3.5.1.84: biuret amidohydrolase EC 3.5.1.85: (S)-N-acetyl-1-phenylethylamine hydrolase EC
List_of_EC_numbers_(EC_3)
"Achieving the birefringence-bandgap trade-off: Hydrogen-bond engineered biuret-cyanurate". Chinese Journal of Structural Chemistry. 44 (10) 100695. Bibcode:2025ChJSC
Giant_birefringence
American scientist
with nephrotic syndrome" (1939, with Chi Che Wang and Ida Genther) "The biuret reaction in the determination of serum proteins" (1940, with H. W. Robinson)
Corinne_Hogden_Robinson
Australian chemist (1929–2008)
crystallography. He received a PhD in 1957 for his work on the structure of biuret hydrate, where he completed most of the calculations for the crystal structure
Hans_Freeman
The following is a partial list of the "E" codes for Medical Subject Headings (MeSH), as defined by the United States National Library of Medicine (NLM)
List_of_MeSH_codes_(E05)
South Korean indie music festival
Jaurim Johnny Royal Cocore Crash Sumun Gosu Ghetto Bombs Nastyona Dabang Biuret Schizo Uhm Project Joly Brothers Band Hooky Club Special Gosu Dr. Core 911
Ssamzi_Sound_Festival
950.058 – allantoin MeSH D02.065.950.117 – biureas MeSH D02.065.950.130 – biuret MeSH D02.065.950.160 – bromisoval MeSH D02.065.950.395 – hydroxyurea MeSH D02
List_of_MeSH_codes_(D02)
travel, tourism, insurance
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travel, tourism, insurance