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Lab technique in biology and chemistry
Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. In this
Flow_cytometry
Measurement of number and characteristics of cells
Cytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count
Cytometry
Data file standard
Flow Cytometry Standard (FCS) is a data file standard for the reading and writing of data from flow cytometry experiments. The FCS specification has traditionally
Flow_Cytometry_Standard
Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing
Flow_cytometry_bioinformatics
Photoacoustic Flow Cytometery
Photoacoustic flow cytometry or PAFC is a biomedical imaging modality that utilizes photoacoustic imaging to perform flow cytometry. A flow of cells passes
Photoacoustic_flow_cytometry
Bone marrow cancer in which lymphocytes are overproduced
suspicion for CLL, and a confirmatory diagnostic test, in particular flow cytometry, should be performed unless clinically unnecessary. To be diagnosed
Chronic_lymphocytic_leukemia
corrected mathematically. The compensation can be done through different flow cytometry software such as Flowjo, Flowlogic, Kaluza etc. The first data compensation
Compensation_(cytometry)
data from the flow cytometer. "General Flow Cytometry Glossary and Cell Cycle Analysis Terminology" The Janis V. Giorgi Flow Cytometry Laboratory, UCLA
Gate_(cytometry)
Mass cytometry application to quantify labeled cell targets
during flow cytometry. Manual gating of this data can be performed as is done for flow cytometry and most of the tools available for flow cytometry analysis
CyTOF
Global biotechnology company
include techniques of sample preparation, cell separation, cell sorting, flow cytometry, spatial biology, cell culture, molecular analysis, clinical applications
Miltenyi_Biotec
Routine laboratory test of blood cells
stream and their properties are measured as they flow past sensors in a technique known as flow cytometry. Hydrodynamic focusing may be used to isolate individual
Complete_blood_count
Cytogenetic technique
populations via the combination of flow cytometry with cytogenetic fluorescence in situ hybridization staining protocols. Flow-FISH is most commonly used to
Flow-FISH
Software package for analyzing flow cytometry data
FlowJo is a software package for analyzing flow cytometry data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard format
FlowJo
Monoclonal antibody
notified when sending such a sample. Daratumumab can also interfere with flow cytometric evaluation of multiple myeloma, causing an apparent lack of plasma
Daratumumab
Medical diagnostic method
A tetramer stain is a flow cytometry procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a given antigen within
Tetramer_assay
Laboratory technique
Mass cytometry is a high-dimensional single-cell analysis technique that integrates flow cytometry with time of flight mass spectrometry. It is used for
Mass_cytometry
Way to sort sperm cells in fertilization
techniques of centrifugation or swim-up. Newly applied methods such as flow cytometry expand the possibilities of sperm sorting and new techniques of sperm
Sperm_sorting
Measurement of cell concentration
significance. Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a narrow stream
Cell_counting
Assay created to determine survival of organs, cells, or tissues
activation of stress pathways using DNA microarrays and protein chips. Flow Cytometry: Automation allows for analysis of thousands of cells per second. As
Viability_assay
Artificial selection of the sex of offspring
to UV light during flow cytometry, X spermatozoa fluoresce brighter than Y- spermatozoa. As the spermatozoa pass through the flow cytometer in single
Sex_selection
Estimating waterborne bacteria abundance
used for counting bacterioplankton include epifluorescence microscopy, flow cytometry, measures of productivity through frequency of dividing cells (FDC)
Bacterioplankton counting methods
Bacterioplankton_counting_methods
To count and identify blood cells at high speed and accuracy
width Platelet mean volume Large platelet cell ratio Platelet criteria Flow cytometry Spectrophotometry Absorption spectroscopy Transmission Turbidimetry
Hematology_analyzer
Blood clot
easily measured using techniques such as fluorescence microscopy and flow cytometry ('flow clotometry'). Mural thrombi form and adhere on the inner wall of
Thrombus
Blood test
differential counts: digital image processing of microscope slides and flow cytometry techniques using light scattering and cell staining. These methods remain
White_blood_cell_differential
Subtype of white blood cell
between T cells and B cells in a peripheral blood smear. Normally, flow cytometry testing is used for specific lymphocyte population counts. This can
Lymphocyte
Group of anti-nuclear antibodies
detection. The beads are run through a flow cell which uses a laser to detect fluorescence. Similar to the flow cytometry method of ANA detection, the MIA uses
Anti-dsDNA_antibodies
Fluorescent dye used to stain DNA
microscopy and immunohistochemistry, often with other fluorophores Flow cytometry to count or sort out cells. An example is the use of Hoechst dyes to
Hoechst_stain
Digital Pathology
Tissue image cytometry or tissue cytometry is a method of digital histopathology and combines classical digital pathology (glass slides scanning and virtual
Tissue_cytometry
Biological cell ceasing to carry out its functions
multiparameter flow- and laser scanning- cytometry. It has been used to describe the real-time changes during cell death, detected by flow cytometry. Programmed
Cell_death
Sperm-meets-egg process
microscopy, flow cytometry or fluorescence microscopy can be used for assessing the shedding of the acrosome or "acrosome reaction" of a sperm sample. Flow cytometry
Acrosome_reaction
scatter. To summarize, flow cytometry alone can be used to gather quantitative data about cell cycle phase distribution, but flow cytometry in coordination with
Cell_synchronization
Academic journal
Cytometry Part A is a peer-reviewed scientific journal covering all aspects of the study of cytometry that was established in 1980. It is the official
Cytometry_Part_A
other. Once hybridized, the microsphere beads are sorted, usually using flow cytometry. This allows for the detection of each of the gene variants from the
Suspension_array_technology
multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A
Optimized Multicolor Immunofluorescence Panel
Optimized_Multicolor_Immunofluorescence_Panel
Disease causing insufficient blood cells of all types
mechanism by the bone marrow against destruction by the immune system. Flow cytometry testing is performed regularly in people with previous aplastic anemia
Aplastic_anemia
Drug discovery technique
Press" Florian, Amy; Lepensky, C; Kwon, O; Sklar, L; Haynes, M (201). "Flow cytometry enbles a high-throughput homogenous fluorecent antibody-binding assay
High-throughput_screening
American marine scientist and curator
and community benefactor. As a scientist, she pioneered the use of flow cytometry to investigate marine phytoplankton and co-founded Bigelow Laboratory
Clarice_Yentsch
Species of single-celled organism
studies have attempted to develop systems for real-time detection via flow cytometry or microscopy of airborne sporangia collected in air samplers. Whilst
Phytophthora_infestans
Blood disease in which red blood cells are attacked by the immune system
sensitivity and specificity.[citation needed] Today, the gold standard is flow cytometry for CD55 and CD59 on white and red blood cells. Based on the levels
Paroxysmal nocturnal hemoglobinuria
Paroxysmal_nocturnal_hemoglobinuria
Form of programmed cell death
exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation (flow cytometry), caspase activation, Bid cleavage, and
Apoptosis
Chemical compound
used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercalates in double-stranded DNA, with a high affinity for GC-rich
7-Aminoactinomycin_D
Medical procedure involving an incision in a vein
anticoagulant) Whole blood: CBC, ESR, Coombs test, platelet antibodies, flow cytometry, blood levels of tacrolimus and cyclosporin Pink K2 EDTA (chelator /
Phlebotomy
Liquid component of blood
such as the determination of the amount of blood cells in blood via flow cytometry. Plasma preparation is quick, as it is not coagulated. Serum sample
Blood_plasma
Method for producing lots of identical antibodies
which bind to the native (non-denaturated) forms of antigen proteins. Flow cytometry screening has been used for primary screening of a large number (~1000)
Hybridoma_technology
Study of disease
including immunofluorescence, immunohistochemistry, electron microscopy, flow cytometry, and molecular-pathologic analysis. One of the greatest challenges of
Pathology
American biotechnology company
and modification-specific antibodies. Reagents are created for use in flow cytometry, proteogenomics, ELISA, immunoprecipitation, Western blotting, immunofluorescence
BioLegend
American life science tools company
American life science tools company that offers analytical mass cytometry systems for flow cytometry and tissue imaging, along with associated assays and reagents
Standard_BioTools
Technology in fluid dynamics
applications include flow cytometry and microfluidic circuits. Contrast agent such as droplets and Microbubbles can be produced in flow focusing microfluidics
Flow_focusing
Canadian biological oceanographer (born 1952)
marine macroecology, ocean surveys of plankton from measurements of flow cytometry, and detection of multi-annual ecological change in marine phytoplankton
William_Li_(oceanographer)
Immunological condition
common laboratory tests including KIT-D816X mutational analysis and flow cytometry analysis seeking co-expression of CD117 and CD25 are also commended
Mast_cell_activation_syndrome
Consortium The Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) is a standard related to flow cytometry which establishes criteria to record
Minimum_information_standard
Device to count and size particles
first cell sorter, and was involved in the early development of flow cytometry. Some flow cytometers continue to utilize the Coulter principle to provide
Coulter_counter
Technique used to enhance visual contrast of specimens observed under a microscope
similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light
Staining
Type of white blood cell
differentiation of monocytes in tissues. They can be identified using flow cytometry or immunohistochemical staining by their specific expression of proteins
Macrophage
Region of repetitive nucleotide sequences on chromosomes
Jong G, Lansdorp PM (December 2006). "Flow cytometry and FISH to measure the average length of telomeres (flow FISH)". Nature Protocols. 1 (5): 2365–76
Telomere
Cell found in connective tissue
as expression of either CD2 or CD25 (confirmed by immunostaining or flow cytometry), are characteristic of primary clonal/monoclonal mast cell activation
Mast_cell
Medical science consortium
of flow cytometry and immunophenotyping. During 2012 both SMEs left the project so it obtained full scientific independence. The goal of EuroFlow consortium
EuroFlow
Type of mass spectrometry that uses an inductively coupled plasma to ionize the sample
to reduce the effect noise on the calculated ratios. In the field of flow cytometry, a new technique uses ICP-MS to replace the traditional fluorochromes
Inductively coupled plasma mass spectrometry
Inductively_coupled_plasma_mass_spectrometry
Growth of bacterial colonies
enumeration (cell counting) by direct and individual (microscopic, flow cytometry), direct and bulk (biomass), indirect and individual (colony counting)
Bacterial_growth
Determine the concentration of a virus
needed] While most flow cytometers do not have sufficient sensitivity,[citation needed] there are a few commercially available flow cytometers that can
Virus_quantification
Chemical compound
(green) / 617 nm (orange-red). Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or
Propidium_iodide
End of an organism's life
multiparameter flow- and laser scanning- cytometry. It has been used to describe the real-time changes during cell death, detected by flow cytometry. The word
Death
Chemical compound
extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.[citation needed] Rhodamine
Rhodamine_B
Percentage of guanine and cytosine in DNA or RNA molecules
heated. The most commonly used protocol for determining GC-ratios uses flow cytometry for large numbers of samples. In an alternative manner, if the DNA or
GC-content
Biochemical technique
used for immunofluorescence, and then analysed by flow cytometry.[citation needed] Flow cytometry has several advantages over IHC including: the ability
Immunostaining
Organelle of sperm cells
positive control when assessing the acrosome reaction of a sperm sample by flow cytometry or fluorescence microscopy. This is usually done after staining with
Acrosome
Boosting the number of red blood cells in the bloodstream
label IgG or IgM-coated RBCs and enhance the detection by flow cytometry The flow cytometry is able to detect minor variance in blood group antigens.
Blood_doping
Central North American species of penstemon
estimated the genome size for 40% of the species in the genus using flow cytometry. Penstemon digitalis has one of the largest genomes of the genus Penstemon
Penstemon_digitalis
American biologist
Professionals. She was the Director of California Institute of Technology's Flow Cytometry and Cell Sorting Shared Resource Laboratory 1982-2024 and also the Lab
Rochelle_Diamond
Process of separating populations of cells
sorting methods. Fluorescence-Activated Cell Sorting is also known as flow cytometry cell sorting, or by the acronym FACS, which is a trademark of Becton
Cell_sorting
Chemical compound
extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 6G usually
Rhodamine_6G
Component of blood aiding in coagulation
restore the geometric parameters of individual measured platelets by flow cytometry. More accurate biophysical models of platelet surface morphology that
Platelet
Parasitic crustacean of fish
665 to 790 Megabase pairs (Mbp) length. Two cytometric techniques, flow cytometry (FCM) and Feulgen image analysis densitometry (FIAD), gave measurements
Salmon_louse
Cancer-focused genetic testing and lab services company
services include cytogenetics, fluorescence in situ hybridization (FISH), flow cytometry, immunohistochemistry, anatomic pathology, and molecular genetics. Headquartered
NeoGenomics
Laboratory near Santa Fe, New Mexico
yields. The laboratory contributed to the early development of the flow cytometry technology. In the 1950s, researcher Mack Fulwyler developed a technique
Los Alamos National Laboratory
Los_Alamos_National_Laboratory
methods". PeerJ Computer Science. 2010. doi:10.7717/peerj-cs.350/table-1. "Flow Cytometry" (PDF). Archived from the original (PDF) on 2018-03-18. Retrieved 2014-06-07
Phycobiliprotein
Membrane-bound extracellular vesicles
RNA detection. Flow cytometry is an optical method to detect exosomes in suspension. Nevertheless, the applicability of flow cytometry to detect single
Exosome_(vesicle)
Genus of flowering plants in the birch family Betulaceae
Europe and North Africa – an analysis based on morphometry, karyology, flow cytometry and microsatellites". Taxon. 66 (3): 567–583. Bibcode:2017Taxon..66
Alder
Species of flowering plant
"Genetic characterisation of olive trees from Madeira Archipelago using flow cytometry and microsatellite markers". Genetic Resources and Crop Evolution. 55
Olive
Study of blood diseases and disorders
often deal with hematopathology; techniques and technologies include flow cytometry studies and immunohistochemistry. In the United States, hematopathology
Hematopathology
Irreversible condensation of chromatin in the nucleus of a dying cell
sample is placed under a light microscope. Fluorescence microscopy and flow cytometry also use staining (fluorescent stains) to target the DNA/nuclear fragments
Pyknosis
Abnormal multiples of one or more chromosomes
may be analyzed by flow cytometry to assess chromosomal abnormalities, such as polyploidy, hypodiploidy, and hyperdiploidy. Flow cytometers have the
Polysomy
Species of plant in the family Fabaceae
2020). "Rooibos (Aspalathus linearis) genome size estimation using flow cytometry and K-Mer analyses". Plants. 9 (2): 270. Bibcode:2020Plnts...9..270M
Rooibos
Type of macrophage
microscopically, but it is also possible to investigate this phenomenon by flow cytometry. LE cells shouldn't be confused with Tart cells which have engulfed
LE_cell
Group of membrane proteins
production of a stable ENaC cell line using fluorogenic signaling probes and flow cytometry to scan numerous cells to isolate rare clones capable of functional
Epithelial_sodium_channel
Blood disease of the fetus and newborn
cells can help determine if a quantitative test (Kleihauer-Betke or flow cytometry) is needed. This is done when exposure is suspected due to a potential
Rh_disease
Annual herb
essential oil: its antibacterial activity and mode of action evaluated by flow cytometry". Journal of Medical Microbiology. 60 (Pt 10): 1479–86. doi:10.1099/jmm
Coriander
A side population (SP) in flow cytometry is a sub-population of cells that is distinct from the main population on the basis of the markers employed. By
Side_population
Branch of biology that studies cells
Approach. 2nd Edition. McKinnon, Katherine M. (21 February 2018). "Flow Cytometry: An Overview". Current Protocols in Immunology. 120 (1): 5.1.1–5.1.11
Cell_biology
Hereditary disease group
rhodamine 123 (green fluorescent compound), which can be measured by flow cytometry. This test is abnormal in patients with chronic granulomatous disease
Chronic_granulomatous_disease
Vesicles found outside cells
successfully for single-EV analysis include optical microscopy and flow cytometry (for large EVs, usually >200 nm), tunable resistive pulse sensing for
Extracellular_vesicle
Form of pathologic analysis
examined by a pathologist under a light microscope and analyzed by flow cytometry, chromosome analysis, or polymerase chain reaction (PCR). Frequently
Bone_marrow_examination
apoptotic and dead cells, and the numbers are then counted using either flow cytometry or a fluorescence microscope. The annexin a5 protein binds to apoptotic
Annexin_A5_affinity_assay
Chemical compound
"New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy". Journal of Immunological Methods. 133
Carboxyfluorescein succinimidyl ester
Carboxyfluorescein_succinimidyl_ester
Chemical compound
extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine fluorescence
Rhodamine_123
British biologist
aspects of fluorescence imaging and flow cytometry. Before starting his position as Head of Imaging and Cytometry at the University of York in 2022, O'Toole
Peter_J._O'Toole
metabolise it into impermeable fluorescent product that can be detected by flow cytometry. This product can’t be formed in metabolically inactive dead cells.
Complement-dependent cytotoxicity
Complement-dependent_cytotoxicity
Detection of protein-protein interactions
Vesicles Using Proximity Ligation Assay with Flow Cytometry Readout—ExoPLA". Current Protocols in Cytometry. 81 (1): 4.8.1–4.8.10. doi:10.1002/cpcy.22.
Proximity_ligation_assay
Type of blood collection equipment for medical testing
anticoagulant) Whole blood: CBC, ESR, Coombs test, platelet antibodies, flow cytometry, blood levels of tacrolimus and cyclosporin Pink K2 EDTA (chelator /
Vacutainer
Abnormal formation of clumps of inflammatory cells (granulomata)
(EBUS FNA). Tissue from biopsy of lymph nodes is subjected to both flow cytometry to rule out cancer and special stains (acid fast bacilli stain and Gömöri
Sarcoidosis
FLOW CYTOMETRY
FLOW CYTOMETRY
Girl/Female
American, German, Latin
Flowering; Flourishing; Flower; Blossom
Girl/Female
Australian, British, Christian, English, German, Latin
Goddess; Peaceful Soul; Form of Florence; Blooming; Flower; Arrow
Boy/Male
Indian, Sanskrit, Tamil, Telugu
Flow
Girl/Female
Arabic, Gujarati, Indian, Muslim, Telugu
River; Flow
Surname or Lastname
English
English : see Flow.
Boy/Male
Gujarati, Hindu, Indian
Flow
Female
English
English variant spelling of French Fleur, or perhaps just a short form of Latin Flora, both FLOR means "flower."
Surname or Lastname
English
English : unexplained; possibly a variant of Flew, a metonymic occupational name for a fisherman, from Middle English flue, denoting a kind of fishing net.
Surname or Lastname
English
English : variant of Clough.English : metonymic occupational name for a nailer, from Old French clou ‘nail’. Compare Clower.Possibly an Americanized spelling of German Klau, a habitational name for someone from Klau near Aachen or Clauen in Lower Saxony, or Glau, a nickname for an astute person, from Old High German, Low German glou, glau ‘circumspect’.
Girl/Female
Hindu
Aspirant, Flow
Surname or Lastname
English and Scottish
English and Scottish : topographic name for someone who lived near a tumulus, mound or hill, Middle English lowe, from Old English hlÄw (see Law 2).Scottish and English : nickname for a short man, from Middle English lah, lowe (Old Norse lágr; the word was adopted first into the northern dialects of Middle English, where Scandinavian influence was strong, and then spread south, with regular alteration of the vowel quality).English and Scottish (of Norman origin) : nickname for a violent or dangerous person, from Anglo-Norman French lou, leu ‘wolf’ (Latin lupus). Wolves were relatively common in Britain at the time when most surnames were formed, as there still existed large tracts of uncleared forest.Scottish : from a pet form of Lawrence. Compare Lowry 1.Americanized spelling of Jewish Lowe.
Girl/Female
Tamil
Shrawani | à®·à¯à®°à®¾à®µà®£à¯€Â
Aspirant, Flow
Shrawani | à®·à¯à®°à®¾à®µà®£à¯€Â
Surname or Lastname
English
English : from Middle English blowe, blaa, bloo ‘pale’, hence a nickname for someone with an exceptionally pale complexion.Americanized spelling of French Bleau.
Boy/Male
Tamil
Music flow
Girl/Female
Native American American Latin
Arrow.
Girl/Female
Latin Spanish
Flower.
Boy/Male
Hindu
Music flow
Boy/Male
British, English
Laurel
Girl/Female
Indian, Telugu
Flow
Girl/Female
Latin American
The mythological Roman goddess of flowers. Diminutive of Florence: From 'florentius' or...
FLOW CYTOMETRY
FLOW CYTOMETRY
Biblical
burning; adoration,extended land
Boy/Male
Scottish
From the cannon's seat.
Boy/Male
Spanish
noble.
Female
Japanese
(秀å) Japanese name HIDEKO means "splendid child."
Girl/Female
Latin
or Selena.
Girl/Female
Arabic, Muslim, Sindhi
Cheerfulness; Joy; Energy; Happiness
Girl/Female
Latin
Juno's surname.
Girl/Female
Arabic, Modern, Muslim
Brave
Biblical
the house of confidence
Girl/Female
Tamil
River starting from blue mountain (Neel Parvat)
FLOW CYTOMETRY
FLOW CYTOMETRY
FLOW CYTOMETRY
FLOW CYTOMETRY
FLOW CYTOMETRY
v. t.
To render slow; to slacken the speed of; to retard; to delay; as, to slow a steamer.
n.
A continuous movement of something abundant; as, a flow of words.
n.
A stream of water or other fluid; a current; as, a flow of water; a flow of blood.
n. pl.
See Flo.
n.
A low, flat mass of floating ice.
pl.
of Flo
v. t.
To put out of breath; to cause to blow from fatigue; as, to blow a horse.
v. i.
To move with a continual change of place among the particles or parts, as a fluid; to change place or circulate, as a liquid; as, rivers flow from springs and lakes; tears flow from the eyes.
v. t.
To clear of contents by forcing air through; as, to blow an egg; to blow one's nose.
n.
The tidal setting in of the water from the ocean to the shore. See Ebb and flow, under Ebb.
v. i.
To proceed; to issue forth; as, wealth flows from industry and economy.
superl.
Moderate; not intense; not inflammatory; as, low heat; a low temperature; a low fever.
v. i.
To rise, as the tide; -- opposed to ebb; as, the tide flows twice in twenty-four hours.
n.
A low-lying piece of watery land; -- called also flow moss and flow bog.
n.
A side or incidental blow; an accidental blow.
superl.
Not loud; as, a low voice; a low sound.
v. i.
To have or be in abundance; to abound; to full, so as to run or flow over; to be copious.
n.
A defect; a fault; as, a flaw in reputation; a flaw in a will, in a deed, or in a statute.
superl.
Not ready; not prompt or quick; dilatory; sluggish; as, slow of speech, and slow of tongue.
v. t.
To form by inflation; to swell by injecting air; as, to blow bubbles; to blow glass.