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Laboratory technique to multiply an RNA sample for study
quantitative RT-PCR or real-time RT-PCR (sometimes even called quantitative real-time RT-PCR), has been variously abbreviated as qRT-PCR, RT-qPCR, RRT-PCR, and
Reverse transcription polymerase chain reaction
Reverse_transcription_polymerase_chain_reaction
Topics referred to by the same term
RT PCR may refer to: Real-time polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) This disambiguation page lists articles
RT_PCR
Laboratory technique of molecular biology
abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR. The acronym "RT-PCR" commonly denotes
Real-time polymerase chain reaction
Real-time_polymerase_chain_reaction
Laboratory technique to multiply a DNA sample for study
transcription PCR (RT-PCR): for amplifying DNA from RNA. Reverse transcriptase reverse transcribes RNA into cDNA, which is then amplified by PCR. RT-PCR is widely
Polymerase_chain_reaction
Diagnostic testing for SARS-CoV-2 virus infection
described as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used. The Minimum
COVID-19_testing
Species of virus
using RT-PCR. Considering the protocol described within this thesis, 0.1 g of plant material is enough for 14 500 separate reactions. During a RT-PCR specific
Potato_virus_Y
Replication of DNA
polymerase is combined with selective PCR amplification to produce many copies of mutant DNA. RT-PCR differs from conventional PCR as it synthesizes cDNA from mRNA
DNA_synthesis
RNA species present outside of the cells in which they were transcribed
(cDNA). The cDNA then serves as a template for PCR amplification. The major benefits of using RT-PCR are its quantitative accuracy in a dynamic range
Extracellular_RNA
Contagious disease caused by SARS-CoV-2
confirmation by rRT-PCR is of limited specificity in identifying COVID‑19. A large study in China compared chest CT results to PCR and demonstrated that
COVID-19
Various uses and techniques of polymerase chain reaction in the laboratory
during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods that use
Variants_of_PCR
Laboratory technique used in molecular biology
produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and
Rapid amplification of cDNA ends
Rapid_amplification_of_cDNA_ends
Modified polymerase chain reaction
of utmost importance in diagnostic applications of PCR or RT-PCR. Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific
Hot_start_PCR
Genus of viruses
reverse transcription PCR (RT-PCR) tests have been developed for rabies but only been used as a confirmatory test. Real-time PCR-based tests which have
Lyssavirus
Protocols for reporting qPCR experiments
claimed to detect measles virus in children with autism through the use of RT-qPCR, but the results proved to be completely unreproducible by other scientists
MIQE
the reaction. RT-LAMP does not require thermal cycles (unlike PCR) and is performed at a constant temperature between 60 and 65 °C. RT-LAMP is used in
Reverse Transcription Loop-mediated Isothermal Amplification
Reverse_Transcription_Loop-mediated_Isothermal_Amplification
Variant of SARS-CoV-2, the virus that causes COVID-19
"spike-gene drop out" for the spike (S) gene in a subset of RT-PCR assays (e.g., TaqPath COVID-19 RT-PCR assay, ThermoFisher). Though existing in a few other
SARS-CoV-2_Alpha_variant
Medical college in the state of Kerala
blood bank. A realtime Reverse Transcriptase -Polymerase Chain Reaction (RT-PCR) lab was set up in the hospital during the beginning stage of COVID-19 pandemic
Government Medical College, Ernakulam
Government_Medical_College,_Ernakulam
Biotechnological procedure
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction
Digital polymerase chain reaction
Digital_polymerase_chain_reaction
Use of polymerase chain reaction to amplify several different DNA
steroid sulfatase gene. In 2008, multiplex-PCR was used for analysis of microsatellites and SNPs. In 2020, RT-PCR multiplex assays were designed that combined
Multiplex polymerase chain reaction
Multiplex_polymerase_chain_reaction
University of Oxford. Retrieved 5 June 2026. "Molecular Diagnostic Tests (RT-PCR) for Ebola Virus Bundibugyo Strain". Africa CDC. Retrieved 10 June 2026
2026_Ebola_epidemic
Human disease
RT-PCR, nested RT-PCR, TaqMan-based real-time RT-PCR, Immunoglobin M antibodies, and Immunoglobin G detection by ELISA. The two methods involving RT-PCR
Kyasanur_Forest_disease
Kingdom. Both were discovered in Karawang, West Java. 3 March 10,946,722 RT-PCR and TCM specimens had been tested from 7,290,849 people. There were 69,631
Timeline of the COVID-19 pandemic in Indonesia (2021)
Timeline_of_the_COVID-19_pandemic_in_Indonesia_(2021)
DNA molecules formed by human agency at a molecular level generating novel DNA sequences
protein products of the recombinant gene can be detected, typically using RT-PCR or western hybridization methods. Gross phenotypic changes are not the norm
Recombinant_DNA
Scientific study of plant diseases
assays such as polymerase chain reaction (PCR), RT-PCR and loop-mediated isothermal amplification (LAMP). Although PCR can detect multiple molecular targets
Plant_pathology
Disease affecting bone remodeling
hybridisation, reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ-RT-PCR for the detection of canine distemper virus RNA in Paget's disease"
Paget's_disease_of_bone
Deadly viral disease, transmitted through animals
samples can, however, reduce the sensitivity and specificity of the FAT. The RT PCR assays proved to be a sensitive and specific tool for routine diagnostic
Rabies
Molecular biology technique
gene product of interest can be used after determination by microarrays or RT-PCR. The RNA samples are most commonly separated on agarose gels containing
Northern_blot
Species of virus
Specific primers have also been designed for molecular WMV detection by RT-PCR. ICTV (22 August 2025). "Taxon Details: Potyvirus citrulli". Retrieved 1
Watermelon_mosaic_virus
Alternative to the polymerase chain reaction
virus performed as well as the best RT-PCR tests, detecting less concentrated samples missed by some PCR tests and an RT-LAMP test. RPA was developed and
Recombinase polymerase amplification
Recombinase_polymerase_amplification
2009–2010 pandemic of swine influenza caused by H1N1 influenza virus
nasopharyngeal, nasal, or oropharyngeal tissue swab from the patient. Real-time RT-PCR is the recommended test as others are unable to differentiate between pandemic
2009_swine_flu_pandemic
known as BA.2.86 or Pirola, arrived in the southern state Kerala with an RT-PCR-positive sample from Karakulam in Thiruvananthapuram district. The Union
COVID-19_pandemic_in_India
Chinese genome sequencing company
antibiotics. In January 2020, BGI Genomics announced its real-time fluorescent RT-PCR kit that helps in identification of SARS-CoV-2 virus that causes COVID-19
BGI_Group
Corruption scandal in Vietnam
Corporation [vi] bribed Vietnamese government officials to sell COVID-19 RT-PCR tests, at heavily inflated prices to provincial health departments and hospitals
Việt_Á_scandal
Viral respiratory infection
population is currently deemed to be significantly low. Diagnosis is by rRT-PCR testing of blood and respiratory samples. There is no specific vaccine or
MERS
Surge of HMPV cases in 2024–2025 (China)
the infection. RT-qPCR methods provide higher sensitivity, and lower probability of contamination compared to the conventional RT-PCR methods, thus it
HMPV seasonal outbreak in China (2024)
HMPV_seasonal_outbreak_in_China_(2024)
Symbiotic penetrative association between a fungus and the roots of a vascular plant
taxonomic constructions of the phylum Glomeromycota. Real-time PCR or quantitative PCR (qPCR), is becoming a well-established method to quickly amplify and
Arbuscular_mycorrhiza
diagnostic test kits. The same day, the first commercial tests were approved, RT-PCR tests from Roche and Thermo Fisher. Another 13 diagnostic products have
COVID-19_pandemic_in_Canada
Human disease caused by paramyxovirus
chain reaction (rRT-PCR) can be used to detect MuV RNA from the first day symptoms appear, declining over the next 8–10 days. rRT-PCR of saliva is typically
Mumps
Type of blood cancer
(9;22) translocation, or have evidence of the translocation by FISH or RT-PCR despite normal routine karyotyping. The small subset of patients without
Chronic_myelogenous_leukemia
DNA polymerases that originate from thermophiles
of a PCR are specifically changed in the course of PCR optimisation. In addition to PCR, thermostable DNA polymerases are also used for RT-PCR variants
Thermostable_DNA_polymerase
Species of rodent
Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT)-PCR and TaqMan RT-PCR". Journal of Clinical Microbiology. 43 (2): 808–812. doi:10.1128/JCM
Wood_mouse
11,959 deaths in the country. In the meantime, 5,804,358 real-time RT-PCR (RT-qPCR) tests have been performed in 40 laboratories across the country. The
COVID-19_pandemic_in_Nepal
Species of mammal
Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT)-PCR and TaqMan RT-PCR". Journal of Clinical Microbiology. 43 (2): 808–812. doi:10.1128/JCM
House_mouse
000 recoveries, and 60,000 COVID-19-related deaths. More than 11.7 million RT-PCR tests and more than 7.3 million rapid antigen tests were processed. An anti-COVID-19
COVID-19_pandemic_in_Romania
Indian politician (born 1957)
quarantine for those entering the territory, and allowed anyone with a negative RT-PCR test to travel to the islands. The territory had its first COVID-19 case
Praful_Khoda_Patel
Infectious disease caused by the Zika virus
transcriptase PCR (RT-PCR) in acutely ill patients. However, the period of viremia can be short and the World Health Organization (WHO) recommends RT-PCR testing
Zika_fever
Rare genetic disorder
If RT-PCR tests result in cDNA showing the absence of an entire exon or exons, then multiplex genomic PCR testing is performed. Multiplex genomic PCR testing
Lesch–Nyhan_syndrome
Measurement in electrophoresis methods
"Relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR): new possibilities for the screening of anti-inflammatory and
Relative_fluorescence_units
British medical testing company, 2015–2024
2020, during the COVID-19 pandemic, it introduced COVID Nudge, a rapid RT-PCR test for COVID-19. The device uses a disposable sample capsule that is placed
DnaNudge
Biochemical process in marine environments
within these rates making it one of the best large scale research methods. PCR has a long standing relationship in genetic and microbiological experiments
Marine_nitrogen_fixation
RNA virus infecting salmonid fish
characteristics. A commonly used molecular biological method of diagnosis is RT-PCR assays. Attempts at controlling IPNV have included the reduction of horizontal
Infectious pancreatic necrosis virus
Infectious_pancreatic_necrosis_virus
Bioinformatics web tool
transcriptase polymerase chain reaction (RT-PCR) experiments, circumventing genomic DNA contamination. RT-PCR is a technique used for the detection of
Webtag
Indian multinational medical device company
and control. During the COVID-19 pandemic in 2020, Meril manufactured its RT-PCR COVID-19 test kit. In June 2020, Meril Diagnostics was among the seven companies
Meril_Life_Sciences
Regular measurement of the viral load of HIV-positive people
Immunodeficiency Virus, Type 1 (HIV-1) Reverse Transcription (RT) Polymerase Chain Reaction (PCR) Assay". Fda.gov. 2007-01-31. Archived from the original on
Viral_load_monitoring_for_HIV
Fetal DNA in the maternal bloodstream
cffDNA from 511 pregnancies were analyzed using quantitative real-time PCR (RT-qPCR). In 401 of 403 pregnancies where maternal blood was drawn at seven weeks
Cell-free_fetal_DNA
Ongoing COVID-19 viral pandemic in Bhutan
April after returning from the Middle East, now tested negative twice by RT-PCR test on 6 May, and was moved from hospital to de-isolation quarantine in
COVID-19_pandemic_in_Bhutan
Gene which maintains basic cellular functions
should be performed before their use in gene expression experiments such as RT-PCR. In 2020, a web-based database of human and mouse housekeeping genes and
Housekeeping_gene
Enzyme which generates DNA
technique called reverse transcription polymerase chain reaction (RT-PCR). The classical PCR technique can be applied only to DNA strands, but, with the help
Reverse_transcriptase
5th Day) to 1 PCR and 1 Self-ATK (PCR 1st day, ATK 5th day). Starting 1 May 2022, fully-vaccinated travellers will NOT require RT-PCR on arrival but
COVID-19_pandemic_in_Thailand
Genus of viruses
isolation from a Korean black goat. Nucleic acid sequence analysis and RT-PCR are used prevalently as detection and genotyping methods of kobuvirus although
Kobuvirus
Genus of viruses
detected in a patient with a reverse transcription polymerase chain reaction (RT-PCR), where even a single virion and its genetic material can be detected. The
Coltivirus
reopened the Philippines to international tourists and stopped requiring RT-PCR tests of fully vaccinated passengers upon arrival. * Country in ASEAN The
Tourism_in_the_Philippines
Study and research of genes
genetic traits. Genetic analyses include molecular technologies such as PCR, RT-PCR, DNA sequencing, and DNA microarrays, and cytogenetic methods such as
Genetic_analysis
collaborators in Europe and Hong Kong, and published on the 23rd. It used rtRT-PCR, and formed the basis of 250,000 kits distributed by the World Health Organization
Development_of_COVID-19_tests
sensitivity. Following that was Reverse Transcription Polymerase Chain Reaction (RT-PCR) (1990), which had high detection sensitivity. Northern Blotting: Northern
MicroRNA_biosensors
Presence of an unwanted element
virulence genes detection reverse-transcription polymerase chain reaction (RT-PCR) and DNA colony hybridization. Pesticide detection and quantification by
Contamination
Indian diplomat (born 1962)
Paracetamol and 2.5 lakh doses of Hydroxychloroquine, more than 30,000 kits of RT-PCR and Covaxin under the Vaccine Maitri initiative. On 4 April 2022, it was
Vinay_Mohan_Kwatra
related ongoing infection dynamics. Whether one or more mutations visible in RT-PCR tests can be used reliably to identify a variant depends on the prevalence
Variants_of_SARS-CoV-2
Species of virus
adults, and people with chronic medical conditions.[medical citation needed] RT-PCR has a sensitivity of 90-95% and a specificity of 98-99%, while LAMP has
Respiratory_syncytial_virus
DNA reverse transcribed from RNA
cDNA is commonly generated from mRNA for gene expression analyses such as RT-qPCR and RNA-seq. mRNA is selectively reverse transcribed using oligo-dT primers
Complementary_DNA
Methods of identifying disease from body odor
similar to or higher than reverse transcription polymerase chain reaction (RT-PCR) and antigen testing procedures. Grandjean et al. trained the dogs to sit
Smell_as_evidence_of_disease
Genus of viruses
continue to be reported in America, Asia, and Europe. Reverse transcription-PCR (RT-PCR) is the most commonly used detection tool for sapovirus because of its
Sapovirus
Chemical compound
Janssen Pharmaceutical. The FDA also approved the therascreen FGFR RGQ RT-PCR Kit, developed by Qiagen Manchester, Ltd., for use as a companion diagnostic
Erdafitinib
Subfield of forensic science
fluid identification obtained from microarray screening and quantitative RT-PCR confirmation". International Journal of Legal Medicine. 124 (3): 217–226
Body_identification
German virologist researching emergent viruses
virologists in Europe and Hong Kong, published a workflow of a real-time PCR (RT-PCR) diagnostic test, which was accepted by the World Health Organization
Christian_Drosten
Public transport terminal in Quezon City, Philippines
2020, the terminal was closed due the COVID-19 pandemic, and served as an RT-PCR COVID-19 saliva testing site, in partnership with the Philippine Red Cross
Araneta_City_Bus_Port
Branch of biology that studies biological systems at the molecular level
variations, like reverse transcription PCR (RT-PCR) for amplification of RNA, and, more recently, quantitative PCR which allow for quantitative measurement
Molecular_biology
are sent for a confirmatory PCR test. The state had also ordered an additional 5 lakh rapid test kits and 1 lakh RT-PCR kits. In late-April, the state
COVID-19 pandemic in Tamil Nadu
COVID-19_pandemic_in_Tamil_Nadu
Species of virus
(September 1995). "Identification of Zucchini Yellow Mosaic Potyvirus by RT-PCR and Analysis of Sequence Variability". Journal of Virological Methods. 55
Zucchini_yellow_mosaic_virus
Indian politician and lawyer
public interest litigation before Supreme Court of India for fixing of RT PCR Test cost at Rs 400 per test while in some states the charges are as high
Ajay_Agarwal
Chemical compound
Red, are commonly used in molecular biology techniques like quantitative RT-PCR and cellular assays. Newer rhodamine derivatives, such as Alexa 594 and
Texas_Red
Viral disease of poultry
infected tissues. RT-PCR (as mentioned above) was designed for the detection of IBDV genome, such as VP1 coding gene, with the possibility of PCR product sequences
Infectious_bursal_disease
Disease outbreaks in Indian state
over on 10 June 2018. Presence of the virus in patients was confirmed with RT-PCR tests conducted at the Manipal Institute of Virology and the National Virology
Nipah virus outbreaks in Kerala
Nipah_virus_outbreaks_in_Kerala
Hydrolysis probe
PMID 11013345. AlleleID - Assay Design for Bacterial Identification 1. TaqMan RT-PCR resources- primer databases, software, protocols Archived 2010-11-25 at
TaqMan
Basic emotion induced by a perceived threat
neuroinflammation and alarm pheromones release in rats was found: real time RT-PCR analysis of rat brain tissues indicated that shocking the footpad of a rat
Fear
Family of viruses
people. A method using real-time RT-PCR, which can detect all human astrovirus genotypes, has been reported. Some RT-qPCR techniques are able to simultaneously
Astrovirus
Rare cancer of the extremities, often near joints or tendon sheaths
Cases Occurring in Neurofibromatosis Type 1 Patients, Using Two Different RT-PCR-Based Methods of Detection". Modern Pathology. 15 (6): 589–92. doi:10.1038/modpathol
Synovial_sarcoma
American health care startup company
officials, offering to supply 50,000 test kits per week. The company's novel RT-PCR-based Curative SARS-CoV-2 Assay received FDA Emergency Use Authorization
Curative_(company)
4103/ijmr.IJMR_2363_20. PMC 8184085. PMID 33818471. Basu, Mohana (2020-06-27). "RT-PCR, antigen, antibody, TrueNAT — all you need to know about the different Covid
Truenat
1993 disease outbreak
the virus. On June 10, reverse transcription polymerase chain reaction (RT-PCR) was used to obtain a DNA sequence 139 base pairs in length from one segment
1993 Four Corners hantavirus outbreak
1993_Four_Corners_hantavirus_outbreak
Former medical product company
enzymes, transfection reagents, nucleotides and primers, products of PCR and RT-PCR, molecular cloning, nucleic acid purification, in vitro transcription
Fermentas
President of the Philippines since 2022
Spain. He has since recovered from the disease after testing negative on a RT-PCR test on May 5, 2020, a month after testing positive for COVID-19. On July
Bongbong_Marcos
Amount of virus found in host tissue
amount of virus. PCR is used to quantify integrated DNA. Reverse transcription polymerase chain reaction (RT-PCR) is a variation of PCR that can be used
Viral_load
Infectious disease caused by poliovirus
further analysed using reverse transcription polymerase chain reaction (RT-PCR) or genomic sequencing to determine the serotype (i.e., 1, 2, or 3), and
Polio
Mela held in Haridwar, India
prevent the spread of COVID during the event, including a mandatory negative RT-PCR test report for the attendees. However, many attendees refused to follow
Haridwar_Kumbh_Mela
2021 film by Ranjith Shankar
above. They start talking due to their boredom. Few days later, Aditi's RT-PCR results come, and she is COVID negative. She leaves for home while Sunny
Sunny_(2021_film)
Family of proteins that form microfilaments
et al. (June 2002). "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes". Genome
Actin
Disease diagnosis using messenger RNA
In early 1992, RT-PCR was applied in PSA gene expression in peripheral blood for early prostate cancer diagnosis. Digital PCR (dPCR) dPCR is a relatively
MRNA-based_disease_diagnosis
Piece of DNA or RNA obtained by amplification chain reactions (PCR, LCR)
the individual assay. Examples include the Amplicor HIV-1 Monitor Assay (RT-PCR), which has the capacity to recognize HIV in plasma; the HIV-1 QT (NASBA)
Amplicon
Manipulation of an organism's genome
protein) are also used. These include northern hybridisation, quantitative RT-PCR, Western blot, immunofluorescence, ELISA, and phenotypic analysis. The new
Genetic_engineering
RT PCR
RT PCR
Male
Hungarian
Hungarian form of German Gerhard, GELLÉRT means "spear strong."
Surname or Lastname
English, North German, and Hungarian (Lampért)
English, North German, and Hungarian (Lampért) : variant of Lambert.
Surname or Lastname
English (Nottinghamshire)
English (Nottinghamshire) : possibly a habitational name from Beard Hall Farm in Derbyshire, named with Old English brerd ‘edge’, ‘hillside’ + hall ‘hall’, ‘manor house’.Possibly an Americanized spelling of German Bärt(h)el (see Bartel).
RT PCR
RT PCR
Boy/Male
Tamil
Quick
Girl/Female
German, Latin
Pure; Little and Womanly; Virgin; Female Version of Charles or Carl
Girl/Female
Tamil
Rajshri | ராஜஷà¯à®°à¯€
Sage like king
Boy/Male
Hindu, Indian, Marathi
Strong; Powerful
Boy/Male
American, Anglo, British, English
Lives by the Holy Spring; Holy Well
Boy/Male
Bengali, Gujarati, Hindu, Indian, Kannada, Marathi
Capable
Girl/Female
Muslim
Prayer
Boy/Male
Indian, Punjabi, Sikh
Artistic Victory
Girl/Female
Tamil
Ratnavali | ரதà¯à®¨à®¾à®µà®²à¯€
A bunch of gems
Girl/Female
Tamil
Madhushri | மதà¯à®·à¯à®°à¯€
Beauty of Spring
RT PCR
RT PCR
RT PCR
RT PCR
RT PCR