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Functional cloning is a molecular cloning technique that relies on prior knowledge of the encoded protein's sequence or function for gene identification
Functional_cloning
Set of methods in molecular biology
DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine. In a conventional molecular cloning experiment, the
Molecular_cloning
Process of producing individual organisms with identical genomes
for being the first mammal cloned from an adult somatic cell. Another example of artificial cloning is molecular cloning, a technique in molecular biology
Cloning
Type of DNA library
recombinant DNA molecule is transferred into E. coli host cell for cloning. Functional cloning Ying, Shao-Yao (2003). Generation of cDNA Libraries: Methods
CDNA_library
Proposed technique to counter hair loss
first companies to begin experimenting with hair cloning was Intercytex. Intercytex tried to clone new hair follicles from the stem cells harvested from
Hair_cloning
Java method for object duplication
reference, not the object. The clone() method provides this missing functionality. Classes that want copying functionality must implement some method to
Clone_(Java_method)
American immunologist
D. E.; Dustin, M. L.; Erickson, H. P.; Springer, T. A. (1989). "Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1"
Timothy_A._Springer
Dense cluser of restriction sites in DNA
can also be used to add new restriction sites to a multiple cloning site. Multiple cloning sites are strategically designed to maximize flexibility while
Multiple_cloning_site
Duplicate of a system
environment. Cloning is a different kind of operation to replicate and backup, in that the cloned environment is both fully functional and separate in
Clone_(computing)
Virkkala. It was born as a functional clone of RemoteAccess BBS (which in turn was a clone of QuickBBS), but extended the functionality with several newer technology
SuperBBS
Concept in mathematical logic
In logic, a functionally complete set of logical connectives or Boolean operators is one that can be used to express all possible truth tables by combining
Functional_completeness
Apple II clones are computers that share functionality with the Apple II line but were not created by Apple. The systems were cloned, both in the United
List_of_Apple_II_clones
Video game that resembles another video game
'copyright infringement' — Sid Meier on his early clones of Space Invaders and Pac-Man, 2021 Cloning a game in digital marketplaces is common. It is hard
Video_game_clone
Small piece of maintainable DNA
been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use. There are many types of cloning vectors
Cloning_vector
Hardware clones of the NES home video game console
(PolyStation), functional computer keyboards designed for "educational" use, and N64/Dreamcast controllers that plugged directly into a TV. Clones relied heavily
Famiclone
Method of creating a cloned embryo by replacing the egg nucleus with a body cell nucleus
clone). Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning
Somatic_cell_nuclear_transfer
Process of re-creating an extinct organism
they have a 'high chance' of cloning a woolly mammoth". PBS NewsHour. Retrieved 23 November 2014. Stone, R. (1999). "Cloning the Woolly Mammoth". Discover
De-extinction
Children's Lego-style construction blocks
clone manufacturers have been challenged in court by Lego. The lawsuits have been mostly unsuccessful, for courts have generally found the functional
Lego_clone
Repeated fragment of computer source code
"Software Clone Detection and Refactoring" (PDF). ISRN Software Engineering. 2013: 1–8. doi:10.1155/2013/129437. Kapser, C.; Godfrey, M.W., ""Cloning Considered
Duplicate_code
2014 hoax
didn’t just doctor screenshots from CNN; they also created fully functional clones of the websites of Louisiana TV stations and newspapers." It was one
Columbian Chemicals Plant explosion hoax
Columbian_Chemicals_Plant_explosion_hoax
Computer running Mac OS not produced by Apple
change prevented most clone manufacturers from shipping the current version of the operating system and effectively ended the Mac cloning program. At the same
Macintosh_clone
Protein-coding gene in the species Homo sapiens
Bennett MK, Molineaux S, Luo Y, Payan DG, Mancebo HS, Wu J (Nov 2001). "Functional cloning of Src-like adapter protein-2 (SLAP-2), a novel inhibitor of antigen
SLA2
Lattice in universal algebra
special case, the lattice implies Post's functional completeness theorem: any set of Boolean operations is functionally complete if and only if it is not a
Post's_lattice
DNA screening technique
and green screening with circular polymerase extension cloning for easy and reliable cloning". Protein Science. 22 (6): 859–864. doi:10.1002/pro.2268
Blue–white_screen
Chemical compound
α-galactosidase (e.g. Mel1 in yeast). In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional β-galactosidase enzyme in a technique
X-gal
Protein-coding gene in humans
Simins AB, Weighardt H, Weidner KM, Weidle UH, Holzmann B (2000). "Functional cloning of ARM-1, an adhesion-regulating molecule upregulated in metastatic
ADRM1
Protein-coding gene in the species Homo sapiens
0827. PMID 8651928. Shyjan AM, Heldin P, Butcher EC, et al. (1996). "Functional cloning of the cDNA for a human hyaluronan synthase". J. Biol. Chem. 271 (38):
HAS1
Method to obtain monoclonal cell populations by serial dilution
Dilution cloning (also called cloning by limiting dilution or simply limiting dilution) is a technique used to derive a monoclonal population from a heterogeneous
Dilution_cloning
Protein-coding gene in the species Homo sapiens
PMID 9373149. Mio T, Yamada-Okabe T, Arisawa M, Yamada-Okabe H (July 2000). "Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine
Phosphoglucomutase_3
DNA construct
(BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. coli. F-plasmids
Bacterial artificial chromosome
Bacterial_artificial_chromosome
Style of object-oriented programming in which inheritance is based on reusing objects
which can then be cloned and extended. Using fruit as an example, a "fruit" object would represent the properties and functionality of fruit in general
Prototype-based_programming
Torrent index site closed 2013
claiming to be dedicated to isoHunt's continuance brought a near-identical clone of the original website online, accessible via isohunt.to. Former staff
IsoHunt
Biological lab technique
analysis, and positional cloning is then used to narrow the candidate region until the gene and its mutations are found. Positional cloning typically involves
Genetic_screen
Class of enzymes
Stockigt J (2004). "Acetyltransfer in natural product biosynthesis--functional cloning and molecular analysis of vinorine synthase". Bioorg. Med. Chem. 12
Vinorine_synthase
Group of identical cells that share a common ancestry
prominent usage is in describing a clone of B cells. The B cells in the body have two important phenotypes (functional forms)—the antibody secreting, terminally
Clone_(cell_biology)
Protein-coding gene in the species Homo sapiens
Rodriguez C, Huang LJ, Son JK, McKee A, Xiao Z, Lodish HF (2001). "Functional cloning of the proto-oncogene brain factor-1 (BF-1) as a Smad-binding antagonist
FOXG1
Plasmid cloning vector
pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. The designation "pUC" is derived from the classical "p"
PUC19
Protein-coding gene in humans
"Identification of a novel transcriptional activator, BSAC, by a functional cloning to inhibit tumor necrosis factor-induced cell death". The Journal
MRTFA
Extinct subspecies of Iberian ibex
subspecies through cloning of preserved tissue from the last surviving female, a living specimen was born in July 2003. The cloned Pyrenean ibex was born
Pyrenean_ibex
Marvel Comics supervillain
lead Mister Sinister clone is not interested in the deal, but is suddenly killed by another Sinister clone who has a functional X-gene, making him a mutant
Mister_Sinister
Protein-coding gene in the species Homo sapiens
NgR was identified by Strittmatter and colleagues using an expression cloning strategy. NgR is implicated in neuronal plasticity and regeneration. Its
Reticulon_4_receptor
Chemical compound
Stockigt J (2004). "Acetyltransfer in natural product biosynthesis--functional cloning and molecular analysis of vinorine synthase". Bioorg. Med. Chem. 12
Vinorine
Protein-coding gene in the species Homo sapiens
"Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions
BRF1_(gene)
Protein-coding gene in the species Homo sapiens
PMID 1769660. Staunton DE, Dustin ML, Springer TA (May 1989). "Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1"
ICAM2
Sound chip
(uses a functionally-identical part labeled Y2404) From Sega: Bank Panic Super Locomotive Sega System 1 Sega System 2 Sega System E – used a clone, SEGA
Texas_Instruments_SN76489
Operating system that behaves similarly to Unix
Coherent (1983), and UniFlex (1985), aimed to provide businesses with the functionality available to academic users of UNIX. When AT&T allowed relatively inexpensive
Unix-like
Operation on mathematical functions
distribution of a function of a random variable Functional decomposition Functional square root Functional equation Higher-order function Infinite compositions
Function_composition
Bioengineering process
Finally, chimeric libraries are generated to retrieve the desired functional clones by shuffling these amplified homologous genes.[page needed] This in
Protein_engineering
Clone of the Apple I
the clone to be produced with cheaper and more widely available components while retaining the functionality of the original. The Replica 1 is a clone of
Replica_1
Molecular biology method for cloning DNA fragments
The Gateway cloning method is a method of molecular cloning invented and commercialized by Invitrogen since the late 1990s, which makes use of the integration
Gateway_Technology
DNA molecules formed by human agency at a molecular level generating novel DNA sequences
methods, such as restriction enzyme/ligase cloning or Gibson assembly. In standard cloning protocols, the cloning of any DNA fragment essentially involves
Recombinant_DNA
Playable AI-generated Minecraft clone
outputs unpredictable changes in scenery and inventory, limiting its functionality as a traditional video game. Critics noted its lack of sound, low frame
Oasis_(Minecraft_clone)
Study of genes found in the environment
propose the idea of cloning DNA directly from environmental samples as early as 1985. This led to the first report of isolating and cloning bulk DNA from an
Metagenomics
Protein-coding gene in the species Homo sapiens
PMC 139241. PMID 12477932. Bogan JS, Hendon N, McKee AE, et al. (2003). "Functional cloning of TUG as a regulator of GLUT4 glucose transporter trafficking". Nature
ASPSCR1
Real-time text-to-speech AI tool
complexity. 15.ai is credited as the first platform to popularize AI voice cloning in memes and content creation. Voice actors and industry professionals
15.ai
Japanese immunologist and Nobel laureate (born 1942)
S2CID 42076631. Wikidata Q34270140. Noma Y; Sideras P; Naito T; et al. (1986). "Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using
Tasuku_Honjo
Purely functional package manager
cross-platform package manager for Unix and Unix-like systems, with its own functional programming language used to configure those systems. Nix was developed
Nix_(package_manager)
Type of random number generator
Peter, Matthias; Schindler, Werner (September 2, 2022). A Proposal for Functionality Classes for Random Number Generators (2.35 DRAFT ed.). Bundesamt für
Non-physical true random number generator
Non-physical_true_random_number_generator
Window management tool for X11
include Komposé (an Exposé clone for KDE3, now abandoned) and Compiz, the latter containing built-in Exposé-style functionality activated by pressing F12
Skippy_(X)
Protein found in humans
National Library of Medicine. Mahnke-Zizelman DK, Sabina RL (Nov 1992). "Cloning of human AMP deaminase isoform E cDNAs. Evidence for a third AMPD gene
AMP_deaminase_2
Manipulation of an organism's genome
some broad definitions of genetic engineering include selective breeding. Cloning and stem cell research, although not considered genetic engineering, are
Genetic_engineering
Computers derived from the IBM PC
expansion cards. Initially such computers were referred to as PC clones, IBM clones or IBM PC clones, but the term "IBM PC compatible" is now a historical description
IBM_PC_compatible
Church in County Wexford, Ireland
Clone Church is a Romanesque medieval church and National Monument in County Wexford, Ireland. Clone Church is located 2.6 km (1.6 mi) south of Ferns,
Clone_Church
List of computer processor instructions
branch is called BRA by Microchip but JR by Hycon), and a small number of functional changes: The H08A only has two FSR/IND registers, not three. The H08A
PIC_instruction_listings
Character in the Marvel Universe
Clone Conspiracy, it revealed he was repeatedly resurrected, killed and resurrected again by the Jackal as the villain tried to perfect a new cloning
Ben_Reilly
Protein-coding gene in the species Homo sapiens
the cloned human alpha 1c subtype". Mol. Pharmacol. 45 (4): 703–8. PMID 8183249. Hirasawa A, Horie K, Tanaka T, et al. (1993). "Cloning, functional expression
Alpha-1A_adrenergic_receptor
Type of hybrid plasmid
Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid
Fosmid
Protein-coding gene in the species Homo sapiens
S2CID 11588492. Wood LS, Tsai TD, Lee KS, Vogeli G (October 1995). "Cloning and functional expression of a human gene, hIRK1, encoding the heart inward rectifier
Kir2.1
Class of enzymes
menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene". Archives
(+)-Menthofuran_synthase
High-level computer programming conceptualization
object-oriented programming that avoids classes and implements inheritance via cloning of instances Data, Context, and Interaction - paradigm that emphasizes
Programming_paradigm
Protein-coding gene in the species Homo sapiens
M, Ogi K, Tanaka H, Nagai Y, Onda H (October 1994). "Molecular cloning and functional expression of a human thyrotropin-releasing hormone (TRH) receptor
Thyrotropin-releasing hormone receptor
Thyrotropin-releasing_hormone_receptor
Field of molecular biology
Functional genomics is a field of molecular biology that attempts to describe gene (and protein) functions and interactions. Functional genomics make
Functional_genomics
File and archive manager for Microsoft Windows
compare, integrated help, and a task switcher for its tools. Its standard functionality can be expanded with macros (which allow scripting) and plugins. Far
Far_Manager
Arrangement of keys on a typographic keyboard
A keyboard layout is any specific physical, visual, or functional arrangement of key switches, legends, or key mapping (respectively) of a keyboard. Layouts
Keyboard_layout
Small DNA molecule within a cell
used bacterial cloning vectors. These cloning vectors contain a site that allows DNA fragments to be inserted, for example a multiple cloning site or polylinker
Plasmid
Protein-coding gene in the species Homo sapiens
Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, et al. (October 2000). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined
MTFP1
Non-Apple computer running macOS
methods because it manipulated Apple's existing Netboot and Imageboot functionalities and behaved as if the system were running from a network disk. It was
Hackintosh
Mammalian protein found in humans
Van Roy N, Speleman F, et al. (May 1994). "The human MCP-3 gene (SCYA7): cloning, sequence analysis, and assignment to the C-C chemokine gene cluster on
CCL7
Protein-coding gene in humans
(March 1995). "Phenylalkylamine Ca2+ antagonist binding protein. Molecular cloning, tissue distribution, and heterologous expression". The Journal of Biological
Emopamil_binding_protein
Bone marrow cell which produces platelets
of the purification of thrombopoietin, none of these efforts led to the cloning of the molecule, the sine qua non of HGF identification. The breakthrough
Megakaryocyte
Chemical compound
Croteau R (2000). "Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli"
10-Deacetylbaccatin
Financial calculator made by Hewlett-Packard
miniature clone named DM-12CC approximating the size of an ID-1 credit card (88 mm × 59 mm × 7 mm). It closely emulates the functionality of the original
HP-12C
Species of true bug
(1998-06-09). "Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-α-bisabolene synthase
Pyrrhocoris_apterus
methods: cloning based and cloning-free based. Fragmented genomic DNA or complementary DNA (cDNA) of interest is cloned into plasmid vectors. The cloning sites
Paired-end_tag
Receptor activated by peptide hormone GLP-1
Wheeler MB, Leng XH, Ligon BB, Rabin DU, et al. (October 1993). "Cloning and functional expression of the human glucagon-like peptide-1 (GLP-1) receptor"
Glucagon-like peptide-1 receptor
Glucagon-like_peptide-1_receptor
object-oriented programming that avoids classes and implements inheritance via cloning of instances Pipeline programming – a simple syntax change to add syntax
Comparison of multi-paradigm programming languages
Comparison_of_multi-paradigm_programming_languages
Collection of the total genomic DNA from a single organism
in functional analysis. One major use of genomic libraries is hierarchichal shotgun sequencing, which is also called top-down, map-based or clone-by-clone
Genomic_library
Computer programming for quantum computers
\rangle } , and is not to be confused with the impossible operation of cloning; the authors claim it is akin to how sharing is modeled in classical languages
Quantum_programming
Chemical compound
Yamaguchi, Yube; Koizumi, Nozomu; Sano, Hiroshi (2003). "Molecular Cloning and Functional Characterization of Three Distinct N -Methyltransferases Involved
Xanthosine
Chemical compound
Croteau R (2000). "Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli"
Baccatin_III
Data protection software for personal users
proprietary backup, imaging, cloning and cybersecurity suite developed by Acronis International GmbH. It can back up files, data, clone storage media and protects
Acronis_True_Image
Semi-automatic rifle
(AMT). As is alluded to in the nomenclature, the Lightning 25/22 is functionally a clone of the Ruger 10/22 with the substitution of a larger 25-round magazine
AMT_Lightning_25/22
Class of ribonucleic acid that is not translated into proteins
non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed
Non-coding_RNA
American nonprofit organization
culturing, genetic sequencing, and advanced reproductive technologies, such as cloning. The toolkit complements traditional conservation practices, such as captive
Revive_&_Restore
also includes officially licensed Tetris sequels, as well as unofficial clones. In 2017 Guinness World Records reported Tetris to be the game with most
List_of_Tetris_variants
Removal of autoreactive B and T cells before they become immunocompetent
occurs after the functional B-cell receptor (BCR) is assembled. If the BCR demonstrates a high affinity attraction to self-antigen then clonal deletion can
Clonal_deletion
Data structure that always preserves the previous version of itself when it is modified
These types of data structures are particularly common in logical and functional programming, as languages in those paradigms discourage (or fully forbid)
Persistent_data_structure
Protein-coding gene in the species Homo sapiens
CM, Burns JE, Danielson PE, Sutcliffe JG (Mar 1993). "Molecular cloning and functional expression of 5-HT1E-like rat and human 5-hydroxytryptamine receptor
5-HT1F_receptor
Early sample or model built to test a concept or process
prototype serves to verify some key functional aspects of the intended design, but usually does not have all the functionality of the final product. A working
Prototype
(Kōhen)" (Japanese: Ψ眠能力でなりすませ！（後編）) "High Expectations on the New, Fully Functional Mascot" Transliteration: "Kinō Manman! Kitai no Nyū Masukotto" (Japanese:
List of The Disastrous Life of Saiki K. episodes
List_of_The_Disastrous_Life_of_Saiki_K._episodes
travel, tourism, insurance
FUNCTIONAL CLONING
FUNCTIONAL CLONING
Boy/Male
Australian, French
Fictional Swordsman; Ambitious and Filled with Religious Aspirations; From Alexander Dumas's Three Musketeers
Boy/Male
American, British, English
Mighty Spearman; The Fictional Character Jorel Father of Superman
Male
Egyptian
, Functionary of the Interior.
Biblical
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Boy/Male
English
The fictional character Jorel father of Superman.
Male
Egyptian
, an Egyptian functionary.
Boy/Male
American, British, English
Mighty Spearman; One who Saves; The Fictional Character Jorel Father of Superman
Boy/Male
English
The fictional character Jorel father of Superman.
Boy/Male
American, Australian, British, English, French
Mighty Spearman; The Fictional Character Jorel Father of Superman
Boy/Male
English
The fictional character Jorel father of Superman.
Male
Egyptian
, a great functionary.
Male
Egyptian
, the son of the functionary Heknofre.
Boy/Male
English
Modern. The fictional character Jorel father of Superman.
Surname or Lastname
English
English : nickname from the animal, Middle English catte ‘cat’. The word is found in similar forms in most European languages from very early times (e.g. Gaelic cath, Slavic kotu). Domestic cats were unknown in Europe in classical times, when weasels fulfilled many of their functions, for example in hunting rodents. They seem to have come from Egypt, where they were regarded as sacred animals.English : from a medieval female personal name, a short form of Catherine.Variant spelling of German and Dutch Katt.
Male
Egyptian
, an Egyptian functionary.
Boy/Male
American, Australian, British, Danish, English, Finnish, French, German, Scandinavian
Farmer; The Fictional Character Jorel Father of Superman; Earth Worker
Male
Egyptian
, a high Egyptian functionary.
Boy/Male
French
Fictional swordsman: (ambitious and filled with religious aspirations) from Alexander Dumas's...
Male
Celtic
, great justiciary, or functionary.
Boy/Male
Buddhist, Indian, Japanese
Mysterious Function
FUNCTIONAL CLONING
FUNCTIONAL CLONING
FUNCTIONAL CLONING
FUNCTIONAL CLONING
FUNCTIONAL CLONING
FUNCTIONAL CLONING
FUNCTIONAL CLONING
travel, tourism, insurance