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Ability of a substance to resist changes in structure under high temperatures
particular protein is responsible for its thermostability. Another type of force that is responsible for thermostability of a protein is the electrostatic interactions
Thermostability
DNA polymerases that originate from thermophiles
introducing Taq polymerase for PCR. The thermostability of Taq polymerase obliviated the need to add a non-thermostable DNA polymerase to the reaction after
Thermostable_DNA_polymerase
Microorganisms which live in water with high temperature and high acidity
rings attached to their lipids that have been shown to increase the thermostability of the membrane. Despite all of the previously mentioned adaptations
Thermoacidophile
Type of animal that produces metabolic heat, but has no specific body temperature
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Mesotherm
Thermostable form of DNA polymerase I used in polymerase chain reaction
with proof-reading ability but a lower optimal temperature and low thermostability. Versions of the polymerase without the 5'-3' exonuclease domain has
Taq_polymerase
Ability of an organism to keep its body temperature within certain boundaries
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Thermoregulation
Organism where internal heating sources are small or negligible
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Ectotherm
System of measuring the instability of a protein under varying conditions
in. For fluorescence-detection size-exclusion chromatography-based thermostability assay (FSEC-TS) the samples are heated in the same manner as in FastPP
Thermal_shift_assay
Chemical property of substances
their applications in DNA replication techniques, such as PCR, where thermostable enzymes are necessary for proper DNA replication. Enzyme function at
Thermolabile
Metabolic system
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Heterothermy
Chemical compound
resistivity at room temperature is 107 Ω·cm. Ytterbium hydride has a high thermostability. Ytterbium hydride can be produced by reacting ytterbium with hydrogen
Ytterbium(II)_hydride
Animal species that can maintain a body temperature higher than their environment
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Warm-blooded
Organism with considerable internal temperature variation
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Poikilotherm
Organisms with a high active metabolism
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Bradymetabolism
Laboratory technique to multiply a DNA sample for study
oligonucleotides that are a complementary sequence to the target DNA region) and a thermostable DNA polymerase. In the first step of PCR, the two strands of the DNA
Polymerase_chain_reaction
Methods to reduce failure and increase the reliability of PCR
properties of the chimeric polymerases TopoTaq and PfuC2 include enhanced thermostability, specificity and resistance to contaminants and inhibitors. They were
Polymerase chain reaction optimization
Polymerase_chain_reaction_optimization
Organism that thrives at relatively high temperatures
"Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability". Microbiology and Molecular Biology Reviews. 65 (1): 1–43. doi:10
Thermophile
Enzyme
100% identical to Pfu DNA polymerase. Pfu DNA polymerase has superior thermostability and proofreading properties compared with Taq DNA polymerase. Unlike
Pfu_DNA_polymerase
Semicrystalline thermoplastic with high mechanical and chemical resistance
and is commonly used to produce high-quality plastic parts that are thermostable and both electrically and thermally insulating. Filled grades of PEEK
Polyether_ether_ketone
Form of thermoregulation by body size
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Gigantothermy
Organism that maintains body temperature largely by heat from internal bodily functions
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Endotherm
Protein-coding gene in humans
phenol sulfotransferase: effect of mutating cys70 on activity and thermostability". Chemico-Biological Interactions. 92 (1–3): 57–66. doi:10.1016/0009-2797(94)90053-1
SULT1A1
Fast parallel proteolysis (FASTpp) is a method to determine the thermostability of proteins by measuring which fraction of protein resists rapid proteolytic
Fast_parallel_proteolysis
Type of antibiotic
basic compound that is most active with an alkaline reaction. It is also thermostable and soluble in water (while insoluble in organic solvents). Neomycin
Neomycin
Species of archaeon
furiosus has many potential industrial applications, owing to its unique thermostable properties. P. furiosus is used in the process of DNA amplification by
Pyrococcus_furiosus
physiologically superior genotypes from within bulks. (C) Membrane thermostability Although resistance to high temperatures involves several complex tolerance
Breeding for heat stress tolerance
Breeding_for_heat_stress_tolerance
Naturally produced monosaccharide
Bacillus licheniformis or Bacillus subtilis (strain MN-385), which are more thermostable than the originally used enzymes. Starting in 1982, pullulanases from
Glucose
Species of bacterium
motility or remain immotile. T. aquaticus has become famous as a source of thermostable enzymes, particularly the Taq DNA polymerase, as described below. Studies
Thermus_aquaticus
Poisonous mushroom (death cap)
poisoning. Amatoxins, the class of toxins found in these mushrooms, are thermostable: they resist changes due to heat and cold, so their toxic effects are
Amanita_phalloides
Protein detection method
3'exonuclease proficient polymerases lowers background noise and hyper thermostable polymerases mediate a simple assay with a natural hot-start reaction
Proximity_extension_assay
Class of polymers
alipharomatic), Aromatic: these are the most used polyimides because of their thermostability. According to the type of interactions between the main chains, polyimides
Polyimide
Low temperature dehydration process
original (PDF) on October 21, 2013. Wilding, Kristen (25 November 2019). "Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free
Freeze_drying
Class of enzymes
thermal cycles (94 °C/80 °C) or 16 hours of cycling.10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity
DNA_ligase
Swedish scientist (born 1943)
catalytic activity with the anticancer prodrug Telcyta and improved thermostability". Cancers. 16 (4): 762. doi:10.3390/cancers16040762. PMC 10887215.
Bengt_Mannervik
Domain of organisms
these harsh conditions. These enzymes have found many uses. For example, thermostable DNA polymerases, such as the Pfu DNA polymerase from Pyrococcus furiosus
Archaea
Harmful effects from ingestion of toxic substances present in a mushroom
methods of preparation for cooking. Some toxins, such as amatoxins, are thermostable and mushrooms containing such toxins will not be rendered safe to eat
Mushroom_poisoning
Engineering thermoplastic polymer
macromolecules, which he characterized as polymers. Due to problems with thermostability, POM was not commercialized at that time. Circa 1952, research chemists
Polyoxymethylene
and cleaving, in which thermostable DNA polymerase elongation and strand slipping generate duplex tandem repeats, and thermostable endonuclease (PspGI)
Polymerase-endonuclease amplification reaction
Polymerase-endonuclease_amplification_reaction
Form of thermoregulation in which an animal shares in the heat production of another
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Kleptothermy
bridges. Mabinlin-2 is the sweet-tasting protein with the highest known thermostability, which is due to the presence of the four disulfide bridges. It has
Mabinlin
Organism tolerant of a wide temperature range
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Eurytherm
Chemical compound
Solvent Black 3 is an azo dye. It is a non-fluorescent, relatively thermostable lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides
Solvent_Black_3
Use of related sequences to construct an ancestral-like gene
minor protein thermostability throughout the phylogeny – leading to an additive effect resulting in 'superior' ancestral thermostability. Experimental
Ancestral sequence reconstruction
Ancestral_sequence_reconstruction
combinations of desirable characteristics encoded in the parent genes such as thermostability and high activity. The potential for DNA shuffling to produce novel
DNA_shuffling
Species of bacterium
isolated. It was found during a survey in search of bacteria that produce thermostable collagenase. The optimum growth temperature for A. sendaiensis is 55 °C
Alicyclobacillus_sendaiensis
Maintaining stable internal body temperature
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Homeothermy
Protein family
absolutely dependent on the presence of lyotropic salts for activity and thermostability. The crystal structure of Ftr, determined to a reveals a homotetramer
Formylmethanofuran—tetrahydromethanopterin N-formyltransferase
Formylmethanofuran—tetrahydromethanopterin_N-formyltransferase
Term used to describe animals with low levels of oxygen consumption
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Bradyaerobic
Pathogenic type of misfolded protein
et al. (March 2014). "Proteolysis of abnormal prion protein with a thermostable protease from Thermococcus kodakarensis KOD1". Applied Microbiology and
Prion
Species of bacterium
Ghorbel-Frikha, Basma; Kanoun, Safia; Nasri, Moncef (2008-05-01). "Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid
Bacillus_licheniformis
Species of archaeon
alpha-amylase from Thermococcus hydrothermalis contributes to its thermostability". Biologia (Bratislava). 65 (3): 408–415. doi:10.2478/s11756-010-0030-x
Thermococcus_hydrothermalis
Method of DNA amplification
that differs from polymerase chain reaction (PCR) in that it involves a thermostable ligase to join two probes or other molecules together which can then
Ligase_chain_reaction
tb03149.x. Outtrup H, Norman BE (1984). "Properties and application of a thermostable maltogenic amylase produced by a strain of Bacillus modified by recombinant-DNA
Glucan 1,4-alpha-maltohydrolase
Glucan_1,4-alpha-maltohydrolase
other polymerase chain reaction processes, the DNA is amplified by the thermostable DNA polymerase: A target region with an internal section of known sequence
Inverse polymerase chain reaction
Inverse_polymerase_chain_reaction
Type of vaccine
LNP formulations or mRNA secondary structures could account for the thermostability differences [between Moderna and BioNtech], many experts suspect both
MRNA_vaccine
Species of bacteria
environments. Additionally, E. aurantiacum has been found to produce a thermostable protease that functions in alkaline environments. Exiguobacterium aurantiacum
Exiguobacterium_aurantiacum
Type of engineered protein
produced without the loss of thermostability. However, decreasing the number of heptad repeats per α-helix reduces the thermostability of the Alphabody by around
Alphabody
Species of virus
well characterised to atomic resolution, and the viral particles are thermostable. The identification of the virus is attributed to Lister and Thresh in
Cowpea_mosaic_virus
Byproduct in PCR formed by primer annealing to itself
primers: a method known as RNase H-dependent PCR (rhPCR), utilizes a thermostable RNase HII to remove a blocking group from the PCR primers at high temperature
Primer_dimer
Category of polymers, in which the monomers are joined together by ester links
increases their glass transition temperature, melting temperature, thermostability, chemical stability, and solvent resistance. Polyesters can also be
Polyester
Protein-coding gene in the species Homo sapiens
members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Two alternatively spliced variants that encode the same
SULT1A2
Biological process
polymerizes partner strands in each direction from these primers using a thermostable DNA polymerase. Repeating this process through multiple cycles amplifies
DNA_replication
Species of bacterium
GC-content of this bacterium is about 69%, this contributes to the thermostability of this bacterium's genome. The two most widely used strains in laboratory
Thermus_thermophilus
Natural explosive eruption of hot water
"Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability". Microbiology and Molecular Biology Reviews. 65 (1): 1–34. doi:10
Geyser
District in Galmudug, Somalia
Kadeppagari, Ravi-Kumar (2012-04-23). "Evidence for the improvement of thermostability of the maltogenic α-amylase ofAspergillus nigerby negative pressure"
Herale
Polymer with a backbone of one composite and random branches of another composite
grafting methods for copolymer synthesis results in materials that are more thermostable than their homopolymer counterparts. There are three methods of synthesis
Graft_polymer
Entropy associated with a molecule's possible conformations
stabilizes the native states. A correlation has been observed between the thermostability of a protein and its proline residue content. Configuration entropy
Conformational_entropy
Extremophile species of bacterium
PMID 26909071. Farci D, Slavov C, Piano D (2018). "Coexisting properties of thermostability and ultraviolet radiation resistance in the main S-layer complex of
Deinococcus_radiodurans
Species of alga
Thermophilic microalgae have gained biotechnological interest as a source for thermostable enzymes and commercial interest as a source for astaxanthin. C. nivalis
Chlamydomonas_nivalis
Organosulfur chemical compound used as a solvent
amplification) or DNA templates with high GC-content (to decrease thermostability). For example, 10% final concentration of DMSO in the PCR mixture with
Dimethyl_sulfoxide
Family of viruses
DNA genomes that infect archaea. The viruses of this family are highly thermostable and can act as a template for site-selective and spatially controlled
Rudiviridae
Arrangement of amino acid sequence
engineered in the laboratory to improve their catalytic activity or thermostability, or to investigate properties of the original protein. Traditional
Circular permutation in proteins
Circular_permutation_in_proteins
Various uses and techniques of polymerase chain reaction in the laboratory
with a cleavable block on the 3' end that is removed by the action of a thermostable RNase HII enzyme. There are several DNA polymerases that are used in
Variants_of_PCR
Taiwanese molecular biologist and neuroscientist (1950–2026)
Klenow fragment of Escherichia coli DNA polymerase I, which was not thermostable. Because each cycle required high-temperature denaturation, the polymerase
Alice_Chien_Chang
Organism requiring a specific ambient temperature
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Stenotherm
Light re-emitted by chlorophyll molecules during return from excited to non-excited states
"Effects of Water Stress on Photosystem II Photochemistry and Its Thermostability in Wheat Plants" (PDF). Journal of Experimental Botany. 50 (336): 1199–1206
Chlorophyll_fluorescence
Study of the effects of temperature on biology
is required for DNA strand separation without denaturing. Use of the thermostable Taq enables running the PCR at high temperature (~60 °C and above), which
Thermobiology
Protein created by joining other proteins into a single polypeptide
hydrophilic interactions in the solute to increase protein solubility. Thermostability: Singular peptides or protein fragments are typically added to reduce
Fusion_protein
Species of archaeon
Aeropyrum pernix L7Ae multifunctional protein and insight into its extreme thermostability". Acta Crystallographica Section F. 69 (Pt 9): 979–988. doi:10.1107/S1744309113021799
Aeropyrum_pernix
Tiwari, Virendra K.; Berkowitz, David B. (2024). "Exploiting Archaeal/Thermostable Enzymes in Synthetic Chemistry: Back to the Future?". ChemCatChem. 16
David_B._Berkowitz
Biological manufacturing process
Mukherjee, Koel; Nigam, Vinod Kumar (2025-04-03). "Improved production of thermostable pectinase from novel Bacillus siamensis (BIOSMNF45) using computational-conventional
Precision_fermentation
Species of bacterium
the high temperature (96 °C) step required to denature DNA. In 2013, a thermostable group II intron reverse transcriptase (TGIRT), GsI-IIC-MRF, from G. stearothermophilus
Geobacillus stearothermophilus
Geobacillus_stearothermophilus
Poikilotherm Homeothermy Heterothermy Stenotherm Eurytherm Thermolabile Thermostability Gigantothermy Kleptothermy Bradymetabolism Tachymetabolism Thermogenesis
Tachyaerobic
Amino acid
Kumar S, Tsai CJ, Nussinov R (March 2000). "Factors enhancing protein thermostability". Protein Engineering. 13 (3): 179–91. doi:10.1093/protein/13.3.179
Lysine
Class of enzymes
these temperatures, and one focus of research has been engineering more thermostable versions of xylose isomerase and the other enzymes in the process. The
Xylose_isomerase
Chemical compound
is produced by calcining zirconium compounds, exploiting its high thermostability. Three phases are known: monoclinic below 1170 °C, tetragonal between
Zirconium_dioxide
Anaerobic, hyperthermophilic archaeon
of P. yayanosii interplay in thermostability. These residues allow for molecular maintenance and increased thermostability through supporting a tightly
Pyrococcus_yayanosii
Species of archaeon
"Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability". Microbiology and Molecular Biology Reviews. 65 (1): 1–43. doi:10
Methanocaldococcus_jannaschii
Family of toxins
toxin in adults. Their swift intestinal absorption coupled with their thermostability leads to rapid development of toxic effects in a relatively short period
Amatoxin
Enzyme of the glycolysis metabolic pathway
3-phosphate dehydrogenase, NAD binding domain determinants of enzyme thermostability observed in the molecular structure of thermus aquaticus d-glyceraldehyde-3-phosphate
Glyceraldehyde 3-phosphate dehydrogenase
Glyceraldehyde_3-phosphate_dehydrogenase
glucose oxidase for analytical application, phytase with increased thermostability and monooxygenase with improved catalytic efficiency using alternative
Sequence saturation mutagenesis
Sequence_saturation_mutagenesis
Proteins that transport oxygen throughout the bodies of some invertebrate animals
T, Hinz HJ, Penz F, Hoff R, Föll R, Decker H (May 1995). "Extreme thermostability of tarantula hemocyanin". FEBS Letters. 364 (1): 9–12. doi:10
Hemocyanin
Species of bacterium
Moriwaki, M; Inamori, Y (February 1993). "Purification and properties of a thermostable chitinase from Streptomyces thermoviolaceus OPC-520". Applied and Environmental
Streptomyces_thermoviolaceus
Class of proteins
binding of carbenicillin to the active site increases the enzyme's thermostability with conformational changes. The first carboxylate group in carbenicillin
Penicillin-binding_proteins
Process which surrounds tiny particles or droplets with a coating
Paunescu, D.; Stark, W. J.; Grass, R. N. (2014). "Magnetically Recoverable, Thermostable, Hydrophobic DNA/Silica Encapsulates and Their Application as Invisible
Micro-encapsulation
Class of enzymes which synthesize nucleic acid chains or polymers
achieve changes from incremental tweaks like higher speed/accuracy/thermostability or major shifts such as conversion of template and product types. All
Polymerase
Structural carbohydrate in the cell walls of land plants and some algae
melt and reform, whereas non-amidated low methoxyl pectins can form thermostable gels that withstand high temperatures; these properties make low methoxyl
Pectin
Species of prokaryote
requires reduced methyl viologen. It is theorized that P. fumarii thermostability is due to the accumulation of unusual organic solutes. One of these
Pyrolobus_fumarii
L; Li, Z; Ni, J; Sheng, D; Shen, Y (September 2007). "A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic
Glycerate_dehydrogenase
Property of solid materials under mechanical stress
secure service in high-temperature environments that the structural thermostability is essential. Therefore, by making the proper choice, suitable ceramic
Creep_(deformation)
THERMOSTABILITY
THERMOSTABILITY
THERMOSTABILITY
THERMOSTABILITY
Boy/Male
Indian, Marathi
King of All Vedas; One who is the Master of All Vedas
Biblical
a basket
Boy/Male
Sikh
Soft, Gentle
Girl/Female
Tamil
Goddess Parvati, Prasanna Lakshmi
Boy/Male
Indian, Telugu
Goddess; Dancer
Boy/Male
Tamil
Lotus
Boy/Male
Australian, Danish, Dutch, French, German, Greek, Latin, Swedish
Farmer; German Form of George; Earth-worker
Girl/Female
American, Christian, French, Gujarati, Hebrew, Hindu, Indian, Kannada, Latin, Malayalam, Marathi, Sanskrit, Sindhi, Tamil, Telugu
Sweat Heart; A Star; Another Name of River Narmada; One who Snares
Girl/Female
Tamil
Mohanadhvani | மோஹநாதவாநீ
Name of a Raga
Boy/Male
Arthurian Legend
The Green Knight.
THERMOSTABILITY
THERMOSTABILITY
THERMOSTABILITY
THERMOSTABILITY
THERMOSTABILITY