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Fluorescence microscopy technique
Light-sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate-to-high optical resolution, but good optical sectioning
Light sheet fluorescence microscopy
Light_sheet_fluorescence_microscopy
Kind of microscopy
Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage
Lattice light-sheet microscopy
Lattice_light-sheet_microscopy
Viewing of objects which are too small to be seen with the naked eye
standard light microscopy and transmission electron microscopy) or by scanning a fine beam over the sample (for example confocal laser scanning microscopy and
Microscopy
Structured-illumination light sheet microscopy
Structured illumination light sheet microscopy (SI-LSM) is an optical imaging technique used for achieving volumetric imaging with high temporal and spatial
Structured illumination light sheet microscopy
Structured_illumination_light_sheet_microscopy
Type of non-invasive imaging technique
to selectively observe the fluorescence of a single molecule. Light sheet fluorescence microscopy: A fluorescence microscopy technique that illuminates
Fluorescence_imaging
Type of statistical analysis
autocorrelation function is different. Light sheet fluorescence microscopy or selective plane imaging microscopy (SPIM) uses illumination that is done
Fluorescence correlation spectroscopy
Fluorescence_correlation_spectroscopy
Optical imaging technique
fluorescence microscopes. In a conventional (i.e., wide-field) fluorescence microscope, the entire specimen is flooded evenly in light from a light source
Confocal_microscopy
Fluorescence microscopy imaging method
point scanning techniques such as laser scanning confocal microscopy) fluorescence microscopy imaging methods that allow obtaining images with a resolution
Photoactivated localization microscopy
Photoactivated_localization_microscopy
microscopy (3PEF) is a high-resolution fluorescence microscopy based on nonlinear excitation effect. Different from two-photon excitation microscopy,
Three-photon_microscopy
File formats for microscopy data
Microscopy file formats are variations of image file formats used to encode results of microscopy acquision events along with metadata. There are hundreds
Microscopy_file_formats
American physicist (born 1960)
Betzig has worked to develop the field of fluorescence microscopy and photoactivated localization microscopy. He was awarded the 2014 Nobel Prize in Chemistry
Eric_Betzig
typically imaged using confocal microscopy, two-photon microscopy, or one of the many variants of light-sheet fluorescence microscopy. Other less commonly used
Tissue_clearing
formalin fixed paraffin embedded tissues. CLARITY 3DISCO Light sheet fluorescence microscopy Susaki, Etsuo A.; Tainaka, Kazuki; Perrin, Dimitri; Kishino
CUBIC
Non-diffractive wave
In light-sheet fluorescence microscopy, non-diffracting (or propagation-invariant) beams have been utilised to produce very long and uniform light-sheets
Bessel_beam
Image processing task
fields in scientific imaging including atomic force microscopy, light sheet fluorescence microscopy, and planetary satellite imaging. The most common image
Image_destriping
Natural emission of light by biological structures
authentic currency. Autofluorescence can be problematic in fluorescence microscopy. Light-emitting stains (such as fluorescently labelled antibodies)
Autofluorescence
Type of high-resolution microscope
for light microscopy. Dark-field microscopy, a different technique that leverages light scattering against a dark background Light sheet fluorescence microscopy
Ultramicroscope
Protein that exhibits bright green fluorescence when exposed to ultraviolet light
fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally
Green_fluorescent_protein
Technique used to enhance visual contrast of specimens observed under a microscope
Often used in fluorescence microscopy for DNA staining, Hoechst stains appear yellow when dissolved in aqueous solutions and emit blue light under UV excitation
Staining
Fluorescent stain
adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used
DAPI
Specialist field of computer science
gene expression, three-dimensional imaging techniques like Light Sheet Fluorescence Microscopy, and Optical Projection (micro)-Computer Tomography. Given
Computational_science
Species of fish
studies. The combination of transparent zebrafish larva, light sheet fluorescence microscopy, and optical calcium indicators such as GCaMP, allow the
Zebrafish
Monomeric red fluorescent protein
be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540 and 590 nm and emits light in the range of 550-650 nm
MCherry
Field of radiation
Kishan (2020-05-15). "Multi-photon attenuation-compensated light-sheet fluorescence microscopy". Scientific Reports. 10 (1): 8090. Bibcode:2020NatSR..10
Airy_beam
Academic journal
2025. doi:10.1038/s41592-024-02565-3. "Method of the Year 2025: electron microscopy-based connectomics". Nature Methods. 23 (1): 1. 2026. doi:10.1038/s41592-025-02988-6
Nature_Methods
Black-box optimization algorithm
example, it was used to help extending the field of view in light sheet fluorescence microscopy. Hans R. G. W. Verstraete, Sander Wahls, Jeroen Kalkman,
DONE
Swedish professor and researcher of molecular biology
conducting research using three-dimensional (3D) imaging with light sheet fluorescence microscopy and tissue clearing to map and characterize intact tumor
Per_Uhlén
environmental effects. Fluorescence microscopy is a technique whereby certain molecules can be excited with one wavelength of light and will emit another
Lipid bilayer characterization
Lipid_bilayer_characterization
Biological membrane structure
is too thin, so researchers often use fluorescence microscopy. A sample is excited with one wavelength of light and observed in another, so that only
Lipid_bilayer
light-switchable molecules designed for in-cell applications and time-resolved techniques to study RNA folding. Light sheet fluorescence microscopy for
Cluster of Excellence Frankfurt Macromolecular Complexes
Cluster_of_Excellence_Frankfurt_Macromolecular_Complexes
Type of camera that can also capture the direction of travel of light rays
one used in their light field camera. The prototype is built around a Nikon Eclipse transmitted light microscope/wide-field fluorescence microscope and standard
Light_field_camera
Emission of secondary X-rays from a material excited by high-energy X-rays
X-ray fluorescence (XRF) is the emission of characteristic "secondary" (or fluorescent) X-rays from a material that has been excited by being bombarded
X-ray_fluorescence
Thin, flat piece of glass onto which a sample is placed to be examined under a microscope
often used when ultraviolet transparency is important, e.g. in fluorescence microscopy. While plain slides are the most common, there are several specialized
Microscope_slide
integrated into the FiJi/ImageJ2/KNIME ecosystem. FiJi KNIME Light sheet fluorescence microscopy Volume rendering Royer, Loïc A; Weigert, Martin; Günther
ClearVolume
Nano-scale semiconductor particles
QD-LED integrated at a scanning microscopy tip was used to demonstrate fluorescence near-field scanning optical microscopy (NSOM) imaging. Quantum dot photodetectors
Quantum_dot
Cloth bearing the alleged image of Jesus
historical documents and works of art, examined the tapes using polarized light microscopy and other physical and chemical techniques. McCrone concluded that
Shroud_of_Turin
Method of making biological tissue transparent
develop new microscopy methods including confocal expansion microscopy, SPIM light sheet microscopy, and CLARITY-optimized light sheet microscopy (COLM).
CLARITY
Chemical compound
extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 6G
Rhodamine_6G
Photonic sciences research institute in Spain
super resolution STORM and STED, Lattice light sheet, Light sheet fluorescence, Raman and SERS, Fluorescence (Confocal, Multispectral Confocal, FRET,
ICFO
Laser microscope used for Raman spectroscopy
the fluorescence of water, media, and buffers can be removed. Consequently, it is suitable to examine proteins, cells and organelles. Raman microscopy for
Raman_microscope
American physicist
Past-President (2020-2021). His research applies quantitative fluorescence microscopy to the study of hormone secretion from the islets of Langerhans
David_W._Piston
sample is imaged using a confocal microscope or by the use of light sheet fluorescence microscopy. The first step is to align the individual images into a
Neural_circuit_reconstruction
Chemical compound
of Congo red as a fluorescence marker for the exoskeleton of small crustaceans and the cuticle of polychaetes". Journal of Microscopy. 238 (2): 95–101
Congo_red
Allotropes of carbon with a cylindrical nanostructure
UV-visible-near infrared fluorescence spectroscopy and absorption spectroscopy, scanning electron microscopy, and transmission electron microscopy. The Canadian
Carbon_nanotube
Property of waves that can oscillate with more than one orientation
Physics Plane of polarization Spin angular momentum of light Optics Depolarizer (optics) Fluorescence anisotropy Glan–Taylor prism Kerr effect Nicol prism
Polarization_(waves)
Physicist
technique RESOLFT, showing that superresolution microscopy can be realized with lower levels of light in living cells and tissues making it more attractive
Ilaria_Testa
American biophysicist (born 1976)
Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy". Journal of the American Chemical Society. 137 (32): 10216–10223
Raghuveer_Parthasarathy
Species of beetle
Frederic; Stelzer, Ernst H. K. (2014-06-01). "Non-invasive long-term fluorescence live imaging of Tribolium castaneum embryos". Development. 141 (11):
Red_flour_beetle
Anomaly in digital graphics or imagery
the specimen. In light microscopy, artifacts may be produced by air bubbles trapped under the slide's cover slip. In electron microscopy, distortions may
Visual_artifact
Cell organelle that processes proteins
abundant in mammalian liver and gonad cells. The ER was observed by light microscopy by Charles Garnier in 1897, who coined the term ergastoplasm. The lacy
Endoplasmic_reticulum
Physical phenomenon of electron resonance
metal sheet become excited by light that is directed to the sheet with a particular angle of incidence, and then travel parallel to the sheet. Assuming
Surface_plasmon_resonance
Large-scale synchrotron facility in Australia
Fluorescence Microscopy (XFM) beamline Macromolecular and Micro crystallography (MX1 and MX2) beamlines (Protein crystallography) Infrared microscopy
Australian_Synchrotron
Reflecting only some colours of light
the polarization of the light. The term dichromatic is also used in this sense. Dichroic Mirrors: Used in fluorescence microscopy and laser systems to separate
Dichroism
Chemical compound
William (1984). "Hydrogenation of Triton X-100 eliminates its fluorescence and ultraviolet light absorption while preserving its detergent properties". Analytical
Triton_X-100
Protein used by single-celled organisms
vertical alpha helixes (PDB ID: 1X0S ). One more small helix is light blue, beta sheet yellow. Bacteriorhodopsin trimer with one retinal molecule in each
Bacteriorhodopsin
Center for materials conservation and technical research
optical microscopy proteomics scanning electron microscopy with X-ray and energy dispersive fluorescence X-ray radiography ultraviolet-visible light spectrophotometry
Museum_Conservation_Institute
Insoluble protein aggregate with a fibrillar morphology
cross-polarized light. Recently, significant enhancement of fluorescence quantum yield of NIAD-4 was exploited to super-resolution fluorescence imaging of
Amyloid
Single-celled algae with silica cell walls
investigations have involved, and been propelled by, diverse approaches including, microscopy, chemistry, biochemistry, material characterisation, molecular biology
Diatom
fluorescence interference contrast microscopy (FLIC) and reflection interference contrast microscopy (RICM) or interferometric scattering microscopy (iSCAT)
Model_lipid_bilayer
Israeli physicist
Gryczynski, Zygmunt K; Gregor, Ingo; Enderlein, Jörg (eds.). "Tilted Light Sheet Microscopy with 3D Point Spread Functions for Single-Molecule Super-Resolution
Yoav_Shechtman
German physicist
infrared) and optical fluorescence spectroscopy and microscopy (single-molecule studies, FRET, FLIM, super-resolution microscopy) to elucidate the structure
Gerd_Ulrich_Nienhaus
Optical quantitative imaging technique
labeling (neither fluorescence, nor a radioactive marker). An illustration of the contrast enhancement is in the Figure for optical microscopy between cross
SEEC_microscopy
techniques in fluorescence microscopy, which allowed for optical sectioning of the specimen (confocal, multiphoton or light sheet microscopy). For the actual
3DISCO
Spectroscopic technique
depends on the wavelength of light, the numerical aperture of the focusing element, and — in the case of confocal microscopy — on the diameter of the confocal
Raman_spectroscopy
Fungus infection of the skin
potassium hydroxide (KOH) microscopy. Advice often given includes: Avoid sharing clothing, sports equipment, towels, or sheets. Wash clothes in hot water
Dermatophytosis
Soft-bodied, aquatic invertebrates
fluorescent reporter for inserted genes and has had a large impact on fluorescence microscopy. The stinging cells used by jellyfish to subdue their prey can
Jellyfish
Yellow food coloring
demonstrated the utility of tartrazine to enable deep-tissue Raman sensing and fluorescence lifetime imaging. Sunset yellow FCF, also known as Yellow 6 Food Standards
Tartrazine
Biomolecule consisting of chains of amino acid residues
2174/138920308785132668. PMC 2904242. PMID 18691124. Yuste R (December 2005). "Fluorescence microscopy today". Nature Methods. 2 (12): 902–904. Bibcode:2005NatCB...2
Protein
(2018), "Single Molecule Imaging in Live Embryos Using Lattice Light-Sheet Microscopy", in Lyubchenko, Yuri L. (ed.), Nanoscale Imaging: Methods and Protocols
Single-particle_tracking
Protein-pigment complex
powerful tool for researchers in fields such as microscopy, microarray analysis and Western blotting. In light of this, it may be beneficial for researchers
Phycoerythrin
Coherent delocalized electron oscillations
across the interface (e.g. a metal-dielectric interface, such as a metal sheet in air). SPs have lower energy than bulk (or volume) plasmons which quantise
Surface_plasmon
Physicist
Confocally-Aligned Planar Excitation (SCAPE) microscopy, which combines light-sheet microscopy and laser scanning confocal microscopy. The technique uses a single objective
Elizabeth_Hillman
Material which glows when excited by ionizing radiation
material). The process then corresponds to one of two phenomena: delayed fluorescence or phosphorescence. The correspondence depends on the type of transition
Scintillator
(American, 1927–2020) — developer of multiphoton microscopy Gregorio Weber (Argentinian, 1916–1997) fluorescence spectroscopy and protein chemistry John Wikswo
List_of_biophysicists
British scientist (1868/70–1949)
between viruses and bacteria. From 1936 he studied fluorescence in micro-organisms under ultraviolet light. From 1920 he studied "filterable viruses", which
Joseph_Edwin_Barnard
American biophysicist
Douglas Smith on single-molecule studies of DNA. She used video fluorescence microscopy and single molecule tracking to monitor the diffusion coefficients
Rae_Robertson-Anderson
Coenzyme
These changes in fluorescence are also used to measure changes in the redox state of living cells, through fluorescence microscopy. NADH can be converted
Nicotinamide adenine dinucleotide
Nicotinamide_adenine_dinucleotide
new microscopy technique known as virus-view dual confocal and AFM (ViViD-AFM), which combines atomic force microscopy with fluorescence microscopy, is
2025_in_science
Deadly viral disease, transmitted through animals
rabies. Some light microscopy techniques may also be used to diagnose rabies at a tenth of the cost of traditional fluorescence microscopy techniques,
Rabies
September – Researchers develop a new method merging confocal fluorescence microscopy with microfluidic laminar flow, that can detect nanoparticles and
2024_in_science
Species of halotolerant archaea
Gronau S, Bolhuis H, Gruska M, Pfeiffer F, Oesterhelt D (2015). "Fluorescence microscopy visualization of halomucin, a secreted 927 kDa protein surrounding
Haloquadratum_walsbyi
Structures projecting from cells
astrocytes. Numerous imaging methods, such as immunohistochemistry and fluorescence microscopy, have enabled the precise targeting of, and are currently used
Cellular_extensions
Combination in the membranes of cells
Resonance (NMR) although fluorescence microscopy remains the dominant technique. In the future it is hoped that super-resolution microscopy such as Stimulated
Lipid_raft
Chemical compound
others) and as a preservative.[citation needed] In fluorescence microscopy and related fluorescence-based techniques, ascorbic acid can be used as an antioxidant
Chemistry_of_ascorbic_acid
Bachilo; Silvio H. Litovsky & R. Bruce Weisman (2004). "Near-Infrared Fluorescence Microscopy of Single-Walled Carbon Nanotubes in Phagocytic Cells". Journal
Optical properties of carbon nanotubes
Optical_properties_of_carbon_nanotubes
Greek physicist
1016/j.giec.2004.03.008. ISSN 1052-5157. PMID 15261200. "Label-free fluorescence microscopy offers early cancer detection". Physics World. 2020-06-25. Retrieved
Irene_Georgakoudi
American scientific instrument manufacturer
on 2018-06-12. Retrieved 2018-06-06. "Bruker Acquires Emerging Light Sheet Microscopy Company LUXENDO". May 8, 2017. Archived from the original on January
Bruker
Hematologic cancer that affects lymphocytes
immunophenotyping (targeting of proteins expressed on cells), flow cytometry, and fluorescence in situ hybridization testing. After a diagnosis and before treatment
Lymphoma
Type of hard and shiny finish
condition include optical and digital microscopy, X-ray radiography and computed tomography, X-ray fluorescence, and spectroscopic techniques such as
Lacquer
Form of electromagnetic radiation
light, which makes it possible to probe structures much smaller than can be seen using a normal microscope. This property is used in X-ray microscopy
X-ray
vasculature via fluorescence microscopy and 3D optical coherence tomography. Researchers at Monash University discovered that a sheet of graphene oxide
Potential applications of graphene
Potential_applications_of_graphene
Electric current produced in living cells
(2009). "Frequency-domain fluorescence lifetime optrode system design and instrumentation without a concurrent reference light-emitting diode". Applied
Developmental_bioelectricity
the registered trademark for a light, strong para-aramid synthetic fiber. Typically it is spun into ropes or fabric sheets that can be used as such or as
Timeline of United States inventions (1946–1991)
Timeline_of_United_States_inventions_(1946–1991)
Method to measure velocities in fluid
size needs to be balanced to scatter enough light to accurately visualize all particles within the laser sheet plane, but small enough to accurately follow
Particle_image_velocimetry
Ultrafine particles of silver between 1 nm and 100 nm in size
size using dynamic light scattering, ultra-small-angle X-ray scattering, atomic force microscopy, and transmission electron microscopy; and size distribution
Silver_nanoparticle
Subfield of condensed matter physics
imaging. However, fluorescence microscopy can be readily applied. Liquid crystals are often probed using polarized light microscopy to determine the ordering
Soft_matter
Medical condition involving uncontrolled growth of skin cells
include photography, dermatoscopy, sonography, confocal microscopy, Raman spectroscopy, fluorescence spectroscopy, terahertz spectroscopy, optical coherence
Skin_cancer
Research and scientific development company
shared the Nobel Prize in Chemistry for his work in super-resolved fluorescence microscopy which he began pursuing while at Bell Labs in the Semiconductor
Bell_Labs
Particle with size less than 100 nm
microscopy are the dominant methods. Because nanoparticles have a size below the diffraction limit of visible light, conventional optical microscopy is
Nanoparticle
Pathogenic type of misfolded protein
brain-derived PrPSc structures have been discovered by cryo-electron microscopy. Another brain-derived fibril structure isolated from humans with
Prion
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