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The principle of solid phase DNA sequencing was described in 1989 based on binding of biotinylated DNA to streptavidin-coated magnetic beads and elution
Solid_phase_sequencing
Nucleic acid methods DNA sequencing Solid-phase extraction DeAngelis, M M; Wang, D G; Hawkins, T L (1995-11-25). "Solid-phase reversible immobilization
Solid-phase reversible immobilization
Solid-phase_reversible_immobilization
Process of determining the nucleic acid sequence
DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is
DNA_sequencing
DNA / RNA sequencing technique
Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Nanopore
Nanopore_sequencing
Method of DNA sequencing
and M. Uhlen.[non-primary source needed] The technique combines solid phase sequencing, and use of streptavidin-coated magnetic beads, a recombinant DNA
Pyrosequencing
DNA sequencing using the concept of massively parallel processing
First-generation sequencing Third-generation sequencing RNA Velocity Nyren, P.; Pettersson, B.; Uhlen, M. (January 1993). "Solid Phase DNA Minisequencing
Massively_parallel_sequencing
DNA sequencing method
complementary to solid support (oligonucleotides on flow cell), the barcode sequence (indices), and the binding site for the sequencing primer. Indices
Illumina_dye_sequencing
Commercial production of peptides
Peptides larger than 8 residues are generally assembled by solid phase chemistry. Solid phase peptide synthesis (SPPS) can be carried out either manually
Custom_peptide_synthesis
Scientific technique used in analysing DNA
technologies such as ABI Solid Sequencing. "Open Source Sequencing". arep.med.harvard.edu. Retrieved 2017-09-17. "After Early-Access Phase, Updated Polonator
Polony_sequencing
Sequencing method for traffic signal phases
In the field of traffic engineering, traffic signal phasing refers to sequencing methods at an intersection such that all movements and users are accommodated
Traffic_signal_phasing
Punctual group of DNA
ratio. Polonies can be generated using several techniques that include solid-phase polymerase chain reaction (PCR) in polyacrylamide gels. However, other
Polony_(biology)
MRNA-based cancer vaccine
automated algorithm integrated with workflow based on massive parallel sequencing of tissue generated from cancer patients. As adjuvant therapy, mRNA-4157
Intismeran_autogene
Sequencing of amino acid arrangement in a protein
Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide. This may serve to identify the
Protein_sequencing
Protein detection method
drug efficacy. Most methods for detecting proteins involve the use of a solid phase for first capturing and immobilizing the protein analyte, where in one
Proximity_extension_assay
Swedish scientist
DNA sequencing. The concept, first described in 1993, depends on several important underlying technologies, including attachment of DNA to solid supports
Mathias_Uhlén
Genetic characteristic of tumorous tissue
genomic sequence, and its measurement has been enabled by next generation sequencing. High TMB and DNA damage repair mutations were discovered to be associated
Tumor_mutational_burden
Compound library-based chemical synthesis method
Rockefeller University, Bruce Merrifield, started investigating the solid-phase synthesis of peptides. Synthesis of peptides in a combinatorial fashion
Combinatorial_chemistry
Single-molecule sequencing technology
Transmission electron microscopy DNA sequencing is a single-molecule sequencing technology that uses transmission electron microscopy techniques. The method
Transmission electron microscopy DNA sequencing
Transmission_electron_microscopy_DNA_sequencing
Cancer-focused genetic testing and lab services company
technical-only service model using FISH. In 2013, the company launched its sequencing platform and was listed on the Nasdaq Stock Market as NEO. In 2014, NeoGenomics
NeoGenomics
Type of activated sludge process for the treatment of wastewater
Sequencing batch reactors (SBR) or sequential batch reactors are a type of activated sludge process for the treatment of wastewater. SBRs treat wastewater
Sequencing_batch_reactor
Sampling and analysis of non-solid biological tissues
liquid biopsy, also known as fluid biopsy or fluid phase biopsy, is the sampling and analysis of non-solid biological tissue, primarily blood. Like traditional
Liquid_biopsy
Tumor or other abnormal growth of tissue
"Intratumor heterogeneity and branched evolution revealed by multiregion sequencing". N. Engl. J. Med. 366 (10): 883–92. doi:10.1056/NEJMoa1113205. PMC 4878653
Neoplasm
Chemical compound
and solid tumors including ovarian cancer, breast cancer, lung cancer. Additionally, an ongoing global multi-center, randomized, open-label, phase 3 study
Navitoclax
Cancer genome sequencing is the whole genome sequencing of a single, homogeneous or heterogeneous group of cancer cells. It is a biochemical laboratory
Cancer_genome_sequencing
massively parallel sequencing ams98 presentation Pascal Mayer patents WO 9844151, WO 9844152, WO 0018957, WO 0246456, WO 03074734 "Solid phase DNA amplification:
Manteia_Predictive_Medicine
Laboratory technique to multiply a DNA sample for study
primers) and Enhanced Solid Phase PCR (where conventional solid phase PCR can be improved by employing high Tm and nested solid support primer with optional
Polymerase_chain_reaction
Technology for screening small molecule compounds
secondary amines, semiconductor devices.), mainly to avoid inconvenient solid phase DNA synthesis and to create easily screenable combinatorial libraries
DNA-encoded_chemical_library
studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics tools
List of RNA-Seq bioinformatics tools
List_of_RNA-Seq_bioinformatics_tools
Chemical synthesis of nucleic acids
opposite, 3' to 5' direction. Currently, the process is implemented as solid-phase synthesis using phosphoramidite method and phosphoramidite building blocks
Oligonucleotide_synthesis
Scientific discipline
reacts with azido-molecules with distinctive vigor. The advances in modern sequencing technologies in the late 1990s allowed scientists to investigate DNA of
Chemical_biology
Process of isolating DNA from cells
relies on the fact that the nucleic acids may bind (adsorption) to the solid phase (silica or other) depending on the pH and the salt concentration of the
DNA_extraction
Single-Cell RNA-Sequencing Method
Drop-Seq is a high-throughput, single-cell RNA sequencing (scRNA-seq) technology used to analyze the mRNA expression of thousands of individual cells by
Drop-Seq
Class of short biopolymers
testing, research, and forensics. Commonly made in the laboratory by solid-phase chemical synthesis, these small fragments of nucleic acids can be manufactured
Oligonucleotide
Method of DNA sequence reconstruction
in order to reconstruct the original sequence. This is needed as DNA sequencing technology might not be able to 'read' whole genomes in one go, but rather
Sequence_assembly
Type of virus
(February 2011). "Phase I clinical study of Seneca Valley Virus (SVV-001), a replication-competent picornavirus, in advanced solid tumors with neuroendocrine
Oncolytic_virus
International research effort on genetic variation
project finished its pilot phase, which was described in detail in a publication in the journal Nature. In 2012, the sequencing of 1092 genomes was announced
1000_Genomes_Project
American geneticist (born 1954)
first direct genomic sequencing method in 1984. Described in that publication were the cyclic application of fluids to a solid phase alternating with imaging
George_Church_(geneticist)
American biochemist and inventor (born 1957)
2002. Optimization of Solid-Phase Combinatorial Synthesis. Yan, B., Czarnik, A.W., Eds.; Wiley: New York, NY, 2002. Solid-Phase Organic Syntheses. Volume
Anthony_Czarnik
DNA sequencing method
CAPP-Seq (cancer personalized profiling by deep sequencing) is a next-generation sequencing based method used to quantify circulating DNA in cancer (ctDNA)
CAPP-Seq
Under-construction passenger rail system in the US
authorized by a 2008 statewide ballot, and is being constructed in two major phases. Phase 1, which is about 494 miles (795 km) long, will connect the Bay Area
California_High-Speed_Rail
sequenced and only an even smaller fraction have been isolated and cultured. Sequencing viruses can be challenging because viruses lack a universally conserved
Viral_metagenomics
Russian therapeutic cancer treatment
Russian Federation, a Phase I study is being carried out within the Russian Federation and recruited 48 adult patients with advanced solid tumors for whom standard
EnteroMix
specific organisms. Using methods such as differential media or genome sequencing, physicians and scientists can observe novel functions in organisms for
Diagnostic_microbiology
Observation of the diversity of tumour cells
as in particular for mutation trees. Section sequencing can be done on multiple portions of a single solid tumour, and the variation in the mutation frequencies
Tumour_heterogeneity
All of the contents of a cell including organelles but excluding the nucleus
integrated network, forming a solid mass (gel). This theory thus proposes that the cytoplasm exists in distinct fluid and solid phases depending on the level
Cytoplasm
Oxygen-free break down of material
2007). "Modelling a sequencing batch reactor to treat the supernatant from anaerobic digestion of the organic fraction of municipal solid waste". Journal
Anaerobic_digestion
Class of large biomolecules essential to all known life
laboratory, through the use of enzymes (DNA and RNA polymerases) and by solid-phase chemical synthesis. Nucleic acids are generally very large molecules
Nucleic_acid
Using robots in biology or chemistry labs
group synthesized 96 peptides on plastic pins coated with a solid support for the solid phase peptide synthesis. This method uses a rectangular block moved
Laboratory_robotics
Laboratory technique in biochemistry
for a particular protein (or group of proteins) are immobilized on a solid-phase substrate such as superparamagnetic microbeads or on microscopic agarose
Immunoprecipitation
Novel genomic technique
selection methods have been developed for ONT long-read sequencing. For example, Solid Phase Reversible Immobilisation (SPRI) size selection has been
Pore-C
Reconstruction of genomes of extinct species
hominin ancestry. Solid-phase target enrichment: in this method microarray and real-time PCR method are used in parallel with shotgun sequencing screening. Whole-genome
Paleogenomics
Natural or synthetic fibers with diameters in the nanometer range
Liquid-liquid separation is usually used to form bicontinuous phase structures while solid-liquid phase separation is used to form crystal structures. The gelation
Nanofiber
with the DNA, and the alkyl chain with the hydrophobic surface of the solid phase. Therefore, when heteroduplexes are partially denaturated by heating
Denaturing high performance liquid chromatography
Denaturing_high_performance_liquid_chromatography
American biologist (born 1938)
DNA sequencing system in 1986. These new instruments had a major impact on the emerging fields of proteomics and genomics. The gas-liquid phase protein
Leroy_Hood
Overview of and topical guide to biochemistry
Gram stain – Surface Plasmon Resonance – Microscale Thermophoresis DNA sequencing Polymerase chain reaction Molecular cloning Northern blotting Southern
Outline_of_biochemistry
Pandemic involving influenza
uses this six-phase classification model: "For the sake of clarification, WHO does not use the old system of 6 phases—that ranged from phase 1 (no reports
Influenza_pandemic
Cancer medication
progression and, in some cases, even reverse it. This was shown in a large phase III clinical trial in which patients who failed imatinib therapy (due to
Sunitinib
spectrometry is determining what phase the sample needs to be in. Different ionization methods require different sample phases. Solid phase samples can be ionized
Sample preparation in mass spectrometry
Sample_preparation_in_mass_spectrometry
Field of study
Single-molecule real-time sequencing (SMRT) is a single-molecule DNA sequencing method. Single-molecule real-time sequencing utilizes a zero-mode waveguide
Epigenomics
Application of mass spectrometry
proteins requires that the proteins in solution or solid state be turned into an ionized form in the gas phase before they are injected and accelerated in an
Protein_mass_spectrometry
Emission of light as a result of a chemical reaction
This is a gas-phase reaction of phosphorus vapor, above the solid, with oxygen producing excited states of (PO)2 and HPO. Another gas phase reaction is
Chemiluminescence
Horizontal work surface
typical system consists of the following: An automated infeed system for sequencing the particle board into production. The CorFab Machine, an automated feed-through
Countertop
click-chemistry based method for generating next generation sequencing libraries for deep-sequencing platforms including Illumina, HiSeq, MiSeq and NextSeq
ClickSeq
Swedish professor of biochemistry
needed] He is known for having developed the pyrosequencing method for DNA sequencing.[according to whom?][not verified in body] Pål Nyrén was born 1955.[where
Pål_Nyrén
Biochemical analysis procedure
to move beyond the use of radioactivity, and instead, use liquid- and solid-phase, competitive, and immunoradiometric assays. As a direct result of these
Ligand_binding_assay
Biological treatment process for wastewater or sewage
often precedes secondary treatment, whereby physical phase separation is used to remove settleable solids. During secondary treatment, biological processes
Secondary_treatment
Israeli biotechnology company
for PVRIG. COM701 is currently being evaluated in a Phase 1 study in patients with advanced solid tumors. The study is designed to evaluate the safety
Compugen_Ltd.
Genus of bacteria
organisms by culture, serology, and small sub-unit rRNA gene and whole-genome sequencing. A recent focus in the sub-discipline of molecular phylogenetics has both
Mycoplasma
Protein-coding gene in humans
cell lung cancer, colorectal cancer, and other solid tumors with KRAS G12C mutations. It continues in phase I and II studies for several cancer types as
KRAS
Chemical compound
commonly used as a base for the deprotection of Fmoc-amino acids used in solid-phase peptide synthesis. Piperidine is listed as a Table II precursor under
Piperidine
Music workstation
- sample-based synthesis and wave sequencing to generate sounds from the multisamples stored on an internal solid state drive. 5) The MS-20EX Legacy
Korg_Nautilus
Medical model that tailors medical practices to the individual patient
confirming disease. For example, personalised techniques such as genome sequencing can reveal mutations in DNA that influence diseases ranging from cystic
Personalized_medicine
Biological method of DNA extraction and purification
purified plasmid can be used for many standard applications, such as sequencing and transfections into cells. Plasmids are almost always purified from
Plasmid_preparation
Autonomous body
barcoding of medicinal and aromatic plants of Nepal, using PCR-based and DNA sequencing based molecular markers, Exploration, molecular and biotechnological characterization
Nepal Academy of Science and Technology
Nepal_Academy_of_Science_and_Technology
Invasion of an organism's body by pathogenic agents
This amplification step is followed by next-generation sequencing or third-generation sequencing, alignment comparisons, and taxonomic classification using
Infection
Regular organic or inorganic structure which supports a regular porous structure
Nanoporous materials consist of a regular organic or inorganic bulk phase in which a porous structure is present. Nanoporous materials exhibit pore diameters
Nanoporous_materials
Chemical compound
"Multicenter Phase I Study of Erdafitinib (JNJ-42756493), Oral Pan-Fibroblast Growth Factor Receptor Inhibitor, in Patients with Advanced or Refractory Solid Tumors"
Erdafitinib
S2CID 11115152. Pederson ER, Warshan D, Rasmussen U (September 2019). "Genome Sequencing of Pleurozium schreberi: The Assembled and Annotated Draft Genome of a
List of sequenced plant genomes
List_of_sequenced_plant_genomes
(Seventh ed.). Hoboken, New Jersey: John Wiley & Sons Inc. p. II.B.7 "Phase Sequencing—Ring Barrier Diagram". ISBN 978-1-118-76228-8. "Traffic Signal Design
Traffic light control and coordination
Traffic_light_control_and_coordination
Genomics centre in Toronto, Canada
Innovation Centre of Genome Canada, with an emphasis on next-generation sequencing (NGS) and bioinformatics support. Research at TCAG focuses on the genetic
Centre_for_Applied_Genomics
Tumors of the nervous or endocrine system within the pancreas
have mutations in mTOR pathway genes, such as TSC2, PTEN and PIK3CA. The sequencing discovery might allow selection of which NETs would benefit from mTOR
Pancreatic neuroendocrine tumor
Pancreatic_neuroendocrine_tumor
Measurement of genetic variations
liquid chromatography (DHPLC) uses reversed-phase HPLC to interrogate SNPs. The key to DHPLC is the solid phase which has differential affinity for single
SNP_genotyping
receptor - sequence (biology) - sequence homology - sequence motif - sequencing - serine - serotonin - serotonin receptor - serpin - sexual reproduction
Index of biochemistry articles
Index_of_biochemistry_articles
Israeli biochemist (1955–2021)
cells, and also became the basis of massive parallel sequencing methods such as 454 sequencing or SOLID, and of digital polymerase chain reaction. Tawfik
Dan_Tawfik
Thin, film-like structure separating two fluids, acting as a selective barrier
"Characteristics of microfiltration membranes in a membrane coupled sequencing batch reactor system". Water Res. 37 (5): 1192–1197. Bibcode:2003WatRe
Membrane
Global health science project
and storage of solid tissues for single-cell RNA sequencing", compared to the typical practice of relying on the average of sequencing multiple cells
Human_Cell_Atlas
Presence of an abnormal number of chromosomes in a cell
cancer cells also have abnormal numbers of chromosomes. About 68% of human solid tumors are aneuploid. Aneuploidy originates during cell division when the
Aneuploidy
Indian defense projects under DRDO
propelled to precisely regulated velocity of 800 km/h using phased discharge of dual solid propellant rocket motors. The canopy fragilisation pattern,
Projects_of_DRDO
Branch of biology that studies biological systems at the molecular level
level. Molecular biologists today have access to increasingly affordable sequencing data at increasingly higher depths, facilitating the development of novel
Molecular_biology
2004 studio album by Taking Back Sunday
Victory Records. The title comes from a line in the opening track, "Set Phasers to Stun." Dismissive of several early songs, they wanted to "grow musically
Where_You_Want_to_Be
example, amino acid profiling, racemisation dating, immunodetection, Edman sequencing, peptide mass fingerprinting, and tandem mass spectrometry have been used
Ancient_protein
Research consortium investigating functional elements in human and model organism DNA
worldwide. Once the pilot phase was completed, the project "scaled up" in 2007, profiting immensely from new-generation sequencing machines. And the data
ENCODE
Chemical compound
Al-Ahmadie H, Scott SN, Janakiraman M, et al. (October 2012). "Genome sequencing identifies a basis for everolimus sensitivity". Science. 338 (6104). New
Everolimus
which bases are added one at a time to a strand of DNA attached to a solid phase. Each base has a protecting group to prevent linkage that is not removed
DNA_construct
and thoroughly mixing the portions The original method is based on the solid-phase synthesis of Merrifield The procedure is illustrated in the figure by
Split_and_pool_synthesis
South African and American doctor (born 1952)
to discuss approaches to fighting cancer, including conducting genomic sequencing of 100,000 patients to create a large database of potential genetic factors
Patrick_Soon-Shiong
Boron compound, a salt of boric acid
heating and then grinding the resulting glasslike solid into a powder. It is a white crystalline solid that dissolves in water to make a basic solution
Borax
monitor fluid mixing and trapping of liquids and can also detect solid and gas phases within microfluidic platforms, an ability that is applicable to the
Droplet-based_microfluidics
Houghten, Richard A. (August 1, 1985). "General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody
Richard_A._Houghten
Tumor originating in adipose (fat) tissue
study, shrank various solid tumor types in patients whose tumors' contained neoplastic cells expressing this antigen. A phase II clinical study has recruited
Liposarcoma
travel, tourism, insurance
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
Male
English
Middle English surname (of Norman French origin) transferred to forename use, CHASE means "hunter."Â
Biblical
nativity; generation;begetter;
Boy/Male
Muslim
Solid structure
Boy/Male
Arabic, Muslim
Solid
Boy/Male
Tamil
Solid redemption
Male
French
French form of Latin Stephanus, STÉPHANE means "crown."
Surname or Lastname
English
English : from Middle English pese ‘pea’, hence a metonymic occupational name for a grower or seller of peas, or a nickname for a small and insignificant person. The word was originally a collective singular (Old English peose, pise, from Latin pisa) from which the modern English vocabulary word pea is derived by folk etymology, the singular having been taken as a plural.Robert and John Pease came from Great Baddow, Essex, England, to Salem, MA, in 1634. In 1644 Robert died, leaving a son (also called Robert) who was apprenticed as a weaver in Salem. By 1646 John Pease was living on Martha’s Vineyard.
Boy/Male
Indian
Solid structure
Surname or Lastname
English
English : metonymic occupational name for a huntsman, or rather a nickname for an exceptionally skilled huntsman, from Middle English chase ‘hunt’ (Old French chasse, from chasser ‘to hunt’, Latin captare).Southern French : topographic name for someone who lived in or by a house, probably the occupier of the most distinguished house in the village, from a southern derivative of Latin casa ‘hut’, ‘cottage’, ‘cabin’.Thomas Chase came to MA from Chesham, Buckinghamshire, England, in the 1640s, and had many prominent descendants. Samuel Chase, born in Somerset Co., MD, in 1741, was one of the first members of the U.S. Supreme Court; Philander Chase, born in Cornish, NH, in 1741 was a prominent Episcopal clergyman, and his nephew Salmon Portland Chase (1808–73), also born in Cornish, was governor of OH, a U.S. senator, and secretary of the U.S. Treasury during the Civil War.
Boy/Male
Muslim
Solid
Surname or Lastname
Spanish and Asturian-Leonese (SolÃs)
Spanish and Asturian-Leonese (SolÃs) : habitational name from SolÃs in Asturies or a similarly named place elsewhere.English : from a medieval personal name bestowed on a child born after the death of a sibling, from Middle English solace ‘comfort’, ‘consolation’. The word also came to have the sense ‘delight’, ‘amusement’, and in some cases the surname may have arisen from a nickname for a playful or entertaining person.
Girl/Female
Muslim
Strong, Solid
Girl/Female
Indian
Strong, Solid
Boy/Male
Muslim
Firm, Solid
Boy/Male
Indian
Solid
Boy/Male
Arabic, Muslim, Parsi
Firm; Solid
Boy/Male
Muslim/Islamic
Solid
Boy/Male
Indian
Firm, Solid
Girl/Female
Biblical
Nativity, generation.
Surname or Lastname
German
German : nickname for a swift runner or a timorous person, from Middle High German, Middle Low German hase ‘hare’.Jewish (Ashkenazic) : ornamental name from German Hase ‘hare’.English : from a Middle English nickname, Hase, from Old English hÄs ‘harsh, raucous, or hoarse voice’.Japanese : usually written with characters meaning ‘long valley’; habitational name from a place in Yamato (now Nara prefecture). Listed in the Shinsen shÅjiroku. Some bearers are descended from the Taira clan; they are found mainly in eastern Japan. Also pronounced Nagaya and Nagatani; the original pronunciation was Hatsuse, meaning ‘beginning of the strait’.
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
SOLID PHASE-SEQUENCING
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